About: Protein composition of tomato spotted wilt virus

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Protein composition of tomato spotted wilt virus
Creator	Francki, R Mohared, N Randles, -4nd, J
Source	Elsevier; Medline; PMC
abstract	Abstract Analysis of the protein composition of tomato spotted wilt virus (TSWV), purified by an improved procedure, by polacrylamide gel electrophoresis, revealed three major structural proteins (of MW 84,000, 50,000, and 29,000d) and a minor one of MW 220,000d. The three major proteins constitute about 98% of the total viral protein and all three were shown to be glycoproteins. One of the major proteins (MW 29,000d) and the minor protein were shown to be associated with subviral particles isolated by treatment of virus with the nonionic detergent Nonidet P-40. Only traces of the other two proteins were detected in the subviral particles. Synthesis of virus-induced proteins in TSWV-infected tobacco leaves was studied by labeling infected and healthy tissue with [3H]and [14C]valine, respectively. The labeled tissues were then fractionated into crude subcellular fractions and protein patterns of healthy and infected tissues were compared by coelectrophoresis on polyacrylamide gels. Only one virus-specific protein (of MW 49,000d) was detected in the virus-enriched fractions; this corresponded with the viral structural protein of MW 50,000d.
has issue date	1973-11-30(xsd:dateTime)
bibo:doi	10.1016/0042-6822(73)90283-3
bibo:pmid	4126840
has license	els-covid
sha1sum (hex)	6f84454ada9a8c94757cc98d9c8aa1fd609ad7f9
schema:url	https://doi.org/10.1016/0042-6822%2873%2990283-3
resource representing a document's title	Protein composition of tomato spotted wilt virus
has PubMed Central identifier	PMC7131514
has PubMed identifier	4126840
schema:publication	Virology
resource representing a document's body	covid:6f84454ada9a8c94757cc98d9c8aa1fd609ad7f9#body_text
is schema:about of	covid:arg/6f84454ada9a8c94757cc98d9c8aa1fd609ad7f9 named entity 'protein' named entity 'virus' named entity 'purified' named entity 'viral protein' named entity 'Tomato Spotted Wilt Virus' named entity 'TSWV' named entity 'protein' named entity 'methanol' named entity 'wrr' named entity 'virus' named entity 'molecular weights' named entity 'sodium phosphate' named entity 'TSWV' named entity 'viral protein' named entity 'detergent' named entity 'virus' named entity '2-mercaptoethanol' named entity 'urea' named entity 'leucine' named entity 'polypeptides' named entity 'inoculation' named entity 'virus' named entity 'virus' named entity 'phosphate buffer' named entity 'molecular weight' named entity 'sodium' named entity 'molecular weight' named entity 'Virus' named entity 'virus' named entity 'nucleic acid' named entity 'protein' named entity 'molecular weights' named entity 'TSWV' named entity 'plant cells' named entity 'glycoproteins' named entity 'viral particle' named entity 'Virus' named entity 'mitochondrial' named entity 'protein synthesis' named entity 'virus' named entity 'sodium phosphate' named entity 'dialysis' named entity 'virus' named entity 'PCi' named entity 'membrane-bound' named entity 'Triton x-100' named entity 'virus' named entity 'virus' named entity 'viral envelope' named entity 'outer envelope' named entity 'glycoprotein' named entity 'virus' named entity 'air pump' named entity 'virus' named entity 'Electron microscopic' named entity 'protein' named entity 'cephaloridine' named entity 'protein' named entity 'chloroplast' named entity 'inoculation' named entity 'inoculation' named entity 'polyacrylamide' named entity 'acetic acid' named entity 'phosphate buffer' named entity 'acetic acid' named entity 'tobacco plants' named entity 'viruses' named entity 'protein' named entity 'nucleic acid' named entity '23,000' named entity 'detergent' named entity 'glycoproteins' named entity 'influenza virus' named entity 'valine' named entity 'chloroplast' named entity 'ion' named entity 'Nonidet P-40' named entity 'Gels'

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