About: Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids

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About: Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids
Creator	Snijder, Joost Veesler, David Walls, Alexandra Burrell, Anika Dosey, Annie Borst, Andrew Kollman, Justin Reddy, Vijay
Source	BioRxiv; MedRxiv
abstract	Single particle cryo-electron microscopy (cryoEM) is becoming widely adopted as a tool for structural characterization of biomolecules at near-atomic resolution. Vitrification of the sample to obtain a dense distribution of particles within a single field of view remains a major bottleneck for the success of such experiments. Here, we describe a simple and cost-effective method to increase the density of frozen-hydrated particles on grids with holey carbon support films. It relies on performing multiple rounds of sample application and blotting prior to plunge freezing in liquid ethane. We show that this approach is generally applicable and significantly increases particle density for a range of samples, such as small protein complexes, viruses and filamentous assemblies. The method is versatile, easy to implement, minimizes sample requirements and can enable characterization of samples that would otherwise resist structural studies using single particle cryoEM.
has issue date	2016-12-15(xsd:dateTime)
bibo:doi	10.1101/094623
has license	medrxiv
sha1sum (hex)	6aa2716c00d89f4b760a9167b15d6203303ae6b9
schema:url	https://doi.org/10.1101/094623
resource representing a document's title	Vitrification after multiple rounds of sample application and blotting improves particle density on cryo-electron microscopy grids
schema:publication	bioRxiv
resource representing a document's body	covid:6aa2716c00d89f4b760a9167b15d6203303ae6b9#body_text
is schema:about of	named entity 'structural' named entity 'prior' named entity 'grids' named entity 'application' named entity 'ROUNDS' named entity 'APPLICATION' named entity 'ETHANE' named entity 'BLOTTING' named entity 'METHOD' named entity 'APPLICATION' named entity 'RESOLUTION' named entity 'SINGLE PARTICLE CRYO-ELECTRON MICROSCOPY' named entity 'SIMPLE' named entity 'INCREASES' named entity 'DENSE' named entity 'SAMPLE' named entity 'PARTICLE' named entity 'STUDIES' named entity 'SAMPLES' named entity 'PROTEIN COMPLEXES' named entity 'ADOPTED' named entity 'PRIOR TO' named entity 'MULTIPLE' named entity 'CHARACTERIZATION' named entity 'APPROACH' named entity 'STRUCTURAL' named entity 'IMPROVES' named entity 'SUCCESS' named entity 'EXPERIMENTS' named entity 'MULTIPLE' named entity 'CRYO-ELECTRON MICROSCOPY' named entity 'DENSITY' named entity 'TOOL' named entity 'CARBON' named entity 'SUPPORT' named entity 'SAMPLE' named entity 'APPLICABLE' named entity 'FIELD OF VIEW' named entity 'DESCRIBE' named entity 'SMALL' named entity 'HYDRATED' named entity 'PARTICLE' named entity 'DISTRIBUTION' named entity 'FILAMENTOUS' named entity 'CRYOEM' named entity 'A MAJOR' named entity 'GENERALLY' named entity 'PERFORMING' named entity 'LIQUID' named entity 'PLUNGE FREEZING' named entity 'DENSITY' named entity 'ENABLE' named entity 'STRUCTURAL CHARACTERIZATION' named entity 'EFFECTIVE' named entity 'NEAR-ATOMIC' named entity 'PARTICLES' named entity 'REQUIREMENTS' named entity 'EASY' named entity 'FILMS' named entity 'BIOMOLECULES' named entity 'USING' named entity 'COST' named entity 'HERE' named entity 'SINGLE' named entity 'VITRIFICATION' named entity 'OBTAIN' named entity 'ROUNDS' named entity 'FROZEN' named entity 'BLOTTING' named entity 'RANGE' named entity 'TO INCREASE' named entity 'VITRIFICATION' named entity 'VIRUSES' covid:arg/6aa2716c00d89f4b760a9167b15d6203303ae6b9 named entity 'performing' named entity 'samples' named entity 'experiments'

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