About: Qualitative and quantitative analysis of glucosinolates and nucleosides in Radix Isatidis by HPLC and liquid chromatography tandem mass spectrometry

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About: Qualitative and quantitative analysis of glucosinolates and nucleosides in Radix Isatidis by HPLC and liquid chromatography tandem mass spectrometry Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Qualitative and quantitative analysis of glucosinolates and nucleosides in Radix Isatidis by HPLC and liquid chromatography tandem mass spectrometry
Creator	Hu, Ping Hu, Xiaomei Li, Youkang Liang, Qionglin Luo, Guoan Wang, Xiuming Wang, Yiming Xie, Yuanyuan
Source	Elsevier; Medline; PMC
abstract	Multi-component fingerprinting and quantitation of the glucosinolates and nucleosides in samples of Radix Isatidis have been carried out using high-performance liquid chromatography with diode-array detection and electrospray ionization tandem mass spectrometry (HPLC–DAD–ESI/MS). Five nucleosides together with one glucosinolate were identified by comparing retention times, ultraviolet spectra, mass spectra and/or empirical molecular formulae of reference compounds. Quantitation of these six compounds was carried out simultaneously by HPLC on a Phenomenex Luna C18 column using gradient elution with methanol and water and detection at 254 nm. All calibration curves were linear (r>0.9994) within test ranges. Limits of detection and quantitation were 0.33 ng and 2.50 ng on column, respectively. Intra- and inter-day precision (as relative standard deviation) for all analytes was <2.19% with recoveries in the range 99.6%–101.8% at three concentration levels. The validated method was successfully applied to fingerprinting and assay of 25 batches of Radix Isatidis sourced from different geographical regions of China. The method is simple and reliable and has potential value in the quality control of Radix Isatidis.
has issue date	2013-10-01(xsd:dateTime)
bibo:doi	10.1016/j.apsb.2013.08.002
bibo:pmid	32288995
has license	no-cc
sha1sum (hex)	69f831364cfb2aeccc57bf2e48d7819794ceb5e4
schema:url	https://doi.org/10.1016/j.apsb.2013.08.002
resource representing a document's title	Qualitative and quantitative analysis of glucosinolates and nucleosides in Radix Isatidis by HPLC and liquid chromatography tandem mass spectrometry
has PubMed Central identifier	PMC7104192
has PubMed identifier	32288995
schema:publication	Acta Pharm Sin B
resource representing a document's body	covid:69f831364cfb2aeccc57bf2e48d7819794ceb5e4#body_text
is schema:about of	named entity 'curves' named entity 'water' named entity 'reference' named entity 'range' named entity 'relative standard deviation' named entity 'nucleosides' named entity 'glucosinolates' named entity 'liquid chromatography' named entity 'NUCLEOSIDES' named entity 'APPLIED' named entity 'RETENTION' named entity 'DAD' named entity 'POTENTIAL' named entity 'REFERENCE' named entity 'HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY' named entity 'TEST' named entity 'GLUCOSINOLATES' named entity 'VALUE' named entity 'RADIX ISATIDIS' named entity 'LEVELS' named entity 'INTER-' named entity 'RECOVERIES' named entity 'TIMES' named entity '254' named entity 'EMPIRICAL' named entity 'RANGE' named entity 'ARRAY' named entity 'HPLC' named entity 'NUCLEOSIDES' named entity 'GRADIENT ELUTION' named entity 'ULTRAVIOLET SPECTRA' named entity 'COMPARING' named entity 'WATER' named entity 'MASS SPECTRA' named entity 'LIMITS OF DETECTION' named entity 'TANDEM MASS SPECTROMETRY' named entity 'STANDARD DEVIATION' named entity 'INTRA' named entity 'MOLECULAR' named entity 'DIODE' named entity 'COLUMN' named entity 'COMPONENT' named entity 'SIMPLE' named entity 'GLUCOSINOLATE' named entity 'FINGERPRINTING' named entity 'THESE' named entity 'RELATIVE' named entity 'ELECTROSPRAY IONIZATION' named entity 'ESI' named entity 'METHOD' named entity 'QUANTITATIVE ANALYSIS' named entity 'HPLC' named entity 'QUALITATIVE' named entity 'LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY' named entity 'CALIBRATION' named entity '0.33' named entity 'HAVE' named entity 'CARRIED' named entity 'QUALITY CONTROL' named entity 'GLUCOSINOLATES' named entity 'SAMPLES' named entity 'DAY' named entity 'MULTI' named entity 'IDENTIFIED BY' named entity 'QUANTITATION' named entity 'SIMULTANEOUSLY' named entity 'LINEAR' named entity 'USING' named entity 'DIFFERENT' named entity 'ANALYTES' named entity 'ASSAY' named entity 'RADIX ISATIDIS' named entity 'VALIDATED' named entity 'LIQUID CHROMATOGRAPHY '

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