About: Application of Molecular Diagnostic Techniques for Viral Testing

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Application of Molecular Diagnostic Techniques for Viral Testing
Creator	Cobo, Fernando
Source	PMC
abstract	Nucleic acid amplification techniques are commonly used currently to diagnose viral diseases and manage patients with this kind of illnesses. These techniques have had a rapid but unconventional route of development during the last 30 years, with the discovery and introduction of several assays in clinical diagnosis. The increase in the number of commercially available methods has facilitated the use of this technology in the majority of laboratories worldwide. This technology has reduced the use of some other techniques such as viral culture based methods and serological assays in the clinical virology laboratory. Moreover, nucleic acid amplification techniques are now the methods of reference and also the most useful assays for the diagnosis in several diseases. The introduction of these techniques and their automation provides new opportunities for the clinical laboratory to affect patient care. The main objectives in performing nucleic acid tests in this field are to provide timely results useful for high-quality patient care at a reasonable cost, because rapid results are associated with improvements in patients care. The use of amplification techniques such as polymerase chain reaction, real-time polymerase chain reaction or nucleic acid sequence-based amplification for virus detection, genotyping and quantification have some advantages like high sensitivity and reproducibility, as well as a broad dynamic range. This review is an up-to-date of the main nucleic acid techniques and their clinical applications, and special challenges and opportunities that these techniques currently provide for the clinical virology laboratory.
has issue date	2012-11-30(xsd:dateTime)
bibo:doi	10.2174/1874357901206010104
bibo:pmid	23248732
has license	cc-by-nc
sha1sum (hex)	68f9d17b3481fc9b2466a48716d7e977ca9bbfad
schema:url	https://doi.org/10.2174/1874357901206010104
resource representing a document's title	Application of Molecular Diagnostic Techniques for Viral Testing
has PubMed Central identifier	PMC3522074
has PubMed identifier	23248732
schema:publication	Open Virol J
resource representing a document's body	covid:68f9d17b3481fc9b2466a48716d7e977ca9bbfad#body_text
is schema:about of	named entity 'COST' named entity 'HIGH SENSITIVITY' named entity 'TECHNIQUES' named entity 'VIRAL CULTURE' named entity 'ILLNESSES' named entity 'PROVIDE' named entity 'NUCLEIC ACID AMPLIFICATION TECHNIQUES' named entity 'TESTS' named entity 'THEIR' named entity 'YEARS' named entity 'TIMELY' named entity 'REVIEW' named entity 'CARE' named entity 'BROAD' named entity 'DISCOVERY' named entity 'LABORATORY' named entity 'REAL-TIME POLYMERASE CHAIN REACTION' named entity 'METHODS' named entity 'LIKE' named entity 'QUALITY' named entity 'USEFUL' named entity 'OBJECTIVES' named entity 'DISEASES' named entity 'NUMBER OF' named entity 'PATIENTS' named entity 'VIRUS DETECTION' named entity 'ROUTE' named entity 'CLINICAL VIROLOGY' named entity '104' named entity 'SEND' named entity 'AMPLIFICATION' named entity 'CLINICAL DIAGNOSIS' named entity 'NET' covid:arg/68f9d17b3481fc9b2466a48716d7e977ca9bbfad named entity 'RESULTS' named entity 'REFERENCE' named entity 'CHALLENGES' named entity 'MANAGE' named entity 'SPECIAL' named entity 'REDUCED' named entity 'PROVIDES' named entity 'ASSOCIATED WITH' named entity 'INTRODUCTION' named entity 'JOURNAL' named entity 'ORDERS' named entity 'OPEN' named entity 'VIROLOGY' named entity 'FIELD' named entity 'BUT' named entity 'RAPID' named entity 'SEROLOGICAL' named entity 'NUCLEIC ACID SEQUENCE' named entity 'DATE' named entity 'QUANTIFICATION' named entity 'CLINICAL LABORATORY' named entity 'AUTOMATION' named entity 'AFFECT PATIENT' named entity 'MAIN' named entity 'DEVELOPMENT' named entity 'PATIENT CARE' named entity 'TO PROVIDE' named entity 'PERFORMING' named entity 'MAJORITY' named entity 'GENOTYPING' named entity 'KIND' named entity 'USE OF' named entity 'AVAILABLE' named entity 'UNCONVENTIONAL' named entity 'NUCLEIC ACID' named entity 'DIAGNOSIS' named entity 'NEW' named entity 'VIRAL DISEASES' named entity 'INCREASE' named entity 'HIGH' named entity 'TECHNOLOGY' named entity 'APPLICATIONS' named entity 'POLYMERASE CHAIN REACTION' named entity 'HAVE' named entity 'USED' named entity 'THESE' named entity 'DYNAMIC RANGE'

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