About: Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy

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About: Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy
Creator	Wang, Lin-Fa Niu, Peihua Tan, Wenjie Huang, Baoying Ye, Fei Cui, Jin Sigmundsson, Kristmundur Anderson, Danielle Kim, So Gong, Jing Lim, Xiao Liu, Weiqiang Tan, Xu Yan, Biao Ye, Qian Zhou, Xuming Zu, Wenhong
Source	BioRxiv
abstract	Bats are responsible for the zoonotic transmission of several major viral diseases including the 2003 SARS outbreak and the ongoing COVID-19 pandemic. While bat genomic sequencing studies have revealed characteristic adaptations of the innate immune system, functional genomic studies are urgently needed to provide a foundation for the molecular dissection of the tolerance of viral infections in bats. Here we report the establishment and screening of genome-wide RNAi library and CRISPR library for the model megabat, Pteropus Alecto. We used the complementary RNAi and CRISPR libraries to interrogate Pteropus Alecto cells for infection with two different viruses, mumps virus and Influenza A virus, respectively. Screening results converged on the endocytosis pathway and the protein secretory pathway as required for both viral infections. Additionally, we revealed a general dependence of the C-1-tetrahydrofolate synthase gene, MTHFD1, for viral replication in bat cells as well as in human cells. MTHFD1 inhibitor carolacton potently blocked replication of several RNA viruses including SARS-CoV-2. Our studies provide a resource for systematic inquiry into the genetic underpinnings of bat biology and a potential target for developing broad spectrum antiviral therapy.
has issue date	2020-03-30(xsd:dateTime)
bibo:doi	10.1101/2020.03.29.014209
has license	biorxiv
sha1sum (hex)	681abe795769a807b043603ae723815aa57b00eb
schema:url	https://doi.org/10.1101/2020.03.29.014209
resource representing a document's title	Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy
schema:publication	bioRxiv
resource representing a document's body	covid:681abe795769a807b043603ae723815aa57b00eb#body_text
is schema:about of	named entity 'animal' named entity 'MERS-CoV' named entity 'MERS-CoV' named entity 'Middle East Respiratory Syndrome' named entity 'lentivirus' named entity 'siRNA' named entity 'Cas9' named entity 'MTHFD1' named entity 'virus' named entity 'virus infection' named entity 'paramyxovirus' named entity 'CRISPR' named entity 'tittering' named entity 'GENE' named entity 'viruses' named entity 'sgRNA' named entity 'cytotoxicity' named entity 'immunofluorescence' named entity 'RNAi' named entity 'cell lines' named entity 'multiplexed' named entity 'protein' named entity 'gene knockdown' named entity 'inosine' named entity 'MTHFD1' named entity 'BLAST' named entity 'dose dependent' named entity 'gene' named entity 'PBS' named entity 'ATPase' named entity 'CRISPR' named entity 'siRNA' named entity 'mumps virus' named entity 'SEC23B' named entity 'influenza' named entity 'influenza A virus' named entity 'antibody' named entity 'serial dilution' named entity 'RNAi' named entity 'RNAi screens' named entity 'viral replication' named entity 'influenza A virus' named entity 'siRNA' named entity 'MTHFD1' named entity 'MTHFD1' named entity 'RNA replication' named entity 'mumps virus' named entity 'RNA replication' named entity 'protein' named entity 'SEC23B' named entity 'Virus' named entity 'MTHFD1' named entity 'antiviral drugs' named entity 'transfection' named entity 'small molecule' named entity 'infection' named entity 'oncogenic' named entity 'ARF4' named entity 'viruses' named entity 'CRISPR' named entity 'Western blotting' named entity 'purine analog' named entity 'bat' named entity 'MTHFD1' named entity 'siRNA' named entity 'cell line' named entity 'protein' named entity 'proton'

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