About: The identification and characterization of nucleic acid chaperone activity of human enterovirus 71 nonstructural protein 3AB

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About: The identification and characterization of nucleic acid chaperone activity of human enterovirus 71 nonstructural protein 3AB Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The identification and characterization of nucleic acid chaperone activity of human enterovirus 71 nonstructural protein 3AB
Creator	Zhang, Qi Yang, Jie Zhou, Xi Hu, Yuanyang Xia, Hongjie Wang, Peipei Tang, Fenfen Zhao, Tianyong
Source	Elsevier; Medline; PMC
abstract	Abstract Human enterovirus 71 (EV71) belongs to the genus Enterovirus in the family Picornaviridae and has been recognized as one of the most important pathogens that cause emerging infectious disease. Despite of the importance of EV71, the nonstructural protein 3AB from this virus is little understood for its function during EV71 replication. Here we expressed EV71 3AB protein as recombinant protein in a eukaryotic expression system and uncovered that this protein possesses a nucleic acid helix-destabilizing and strand annealing acceleration activity in a dose-dependent manner, indicating that EV71 3AB is a nucleic acid chaperone protein. Moreover, we characterized the RNA chaperone activity of EV71 3AB, and revealed that divalent metal ions, such as Mg2+ and Zn2+, were able to inhibit the RNA helix-destabilizing activity of 3AB to different extents. Moreover, we determined that 3B plus the last 7 amino acids at the C-terminal of 3A (termed 3B+7) possess the RNA chaperone activity, and five amino acids, i.e. Lys-80, Phe-82, Phe-85, Tyr-89, and Arg-103, are critical and probably the active sites of 3AB for its RNA chaperone activity. This report reveals that EV71 3AB displays an RNA chaperone activity, adds a new member to the growing list of virus-encoded RNA chaperones, and provides novel knowledge about the virology of EV71.
has issue date	2014-09-30(xsd:dateTime)
bibo:doi	10.1016/j.virol.2014.07.037
bibo:pmid	25113906
has license	els-covid
sha1sum (hex)	654a38d9d63fca45d46b698091a1e5579ae07878
schema:url	https://doi.org/10.1016/j.virol.2014.07.037
resource representing a document's title	The identification and characterization of nucleic acid chaperone activity of human enterovirus 71 nonstructural protein 3AB
has PubMed Central identifier	PMC7112070
has PubMed identifier	25113906
schema:publication	Virology
resource representing a document's body	covid:654a38d9d63fca45d46b698091a1e5579ae07878#body_text
is schema:about of	named entity 'amino acids' named entity 'expression system' named entity 'manner' named entity 'virology' named entity 'The' named entity 'acid' named entity 'human' named entity 'CHAPERONE ACTIVITY' named entity 'PROVIDES' named entity 'REVEALED' named entity 'UNDERSTOOD' named entity 'HUMAN ENTEROVIRUS 71' named entity 'LITTLE' named entity 'NEW' named entity 'FUNCTION' named entity 'PATHOGENS' named entity 'HELIX' named entity 'HERE' named entity 'AMINO ACIDS' named entity 'IMPORTANT' named entity 'DIVALENT METAL' named entity 'IS A' named entity 'RECOMBINANT PROTEIN' named entity 'NUCLEIC ACID CHAPERONE' named entity 'CHARACTERIZATION' named entity 'HUMAN ENTEROVIRUS 71' named entity 'NOVEL' named entity 'REPLICATION' named entity 'ACTIVITY' named entity 'POSSESSES' named entity 'MEMBER' named entity 'NUCLEIC ACID CHAPERONE' named entity 'C-TERMINAL' named entity 'VIRUS' named entity 'LIST' named entity 'FAMILY PICORNAVIRIDAE' named entity 'EXTENTS' named entity 'DOSE-DEPENDENT' named entity 'ITS' named entity 'TO INHIBIT' named entity 'ENCODED' named entity 'NUCLEIC ACID' named entity 'EXPRESSED' named entity 'VIROLOGY' named entity 'IDENTIFICATION' named entity 'PROTEIN ' named entity 'CHARACTERIZED' named entity 'EUKARYOTIC' named entity 'PROBABLY' named entity 'DIFFERENT' named entity 'KNOWLEDGE' named entity 'CRITICAL' named entity 'ABOUT' named entity 'EXPRESSION SYSTEM' named entity 'UNCOVERED' named entity 'RNA CHAPERONE' named entity 'STRAND' named entity 'CHAPERONE ACTIVITY' named entity 'ACCELERATION' named entity 'POSSESS' named entity 'REPORT' named entity 'CAUSE' named entity 'GENUS ENTEROVIRUS' named entity 'DISPLAYS' named entity 'RNA' named entity 'ANNEALING' named entity 'ACTIVE SITES' named entity 'PROTEIN ' named entity 'INDICATING' named entity 'GROWING' named entity 'METAL IONS' named entity 'DETERMINED' named entity 'EMERGING INFECTIOUS DISEASE' named entity 'CHAPERONE PROTEIN'

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