About: Direct detection of rhinoviruses by an enzyme‐linked immunosorbent assay

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Direct detection of rhinoviruses by an enzyme‐linked immunosorbent assay
Creator	Al-Nakib, W Dearden, C
Source	PMC
abstract	This paper describes the first enzyme‐linked immunosorbent assay for the detection of rhinovirus antigens in clinical specimens (nasal washings), either directly or following overnight cell culture amplification. The assay takes approximately 48 hours to perform and utilizes the same rabbit antirhinovirus hyperimmune serum as both the capture and detecting antibody. The latter has been biotin‐labelled and is detected via a streptavidin 3‐galactosidase preformed complex. This new assay has been found to be very sensitive, detecting human rhinovirus (HRV)‐EL and HRV‐2 at titres as low as 10(1.8) TCID(50) 100 μl(−1) and < 10(1) TCID(50) 100 μl(−1), respectively. Furthermore, when 57 different human rhinovirus serotypes were tested in both the HRV‐EL and HRV‐2 ELISA systems a total of 49 (86%) were found to be cross‐reactive. Of 36 clinical specimens tested by virus isolation, cell‐culture‐amplified (CCA) ELISA, and direct ELISA, 15 were positive by isolation, 11 by CCA‐ELISA, and 11 by direct ELISA. The overall correlation of the CCA and direct ELISA techniques with virus isolation was found to be 88.9% and 66.7%, respectively. The present study demonstrates that the ELISA system developed is a sensitive technique for the diagnosis of rhinovirus infections.
has issue date	2005-12-07(xsd:dateTime)
bibo:doi	10.1002/jmv.1890230211
bibo:pmid	2824684
has license	no-cc
sha1sum (hex)	650facc5fdb5d1fd898d60bba49ea3c06e2a5bd8
schema:url	https://doi.org/10.1002/jmv.1890230211
resource representing a document's title	Direct detection of rhinoviruses by an enzyme‐linked immunosorbent assay
has PubMed Central identifier	PMC7166933
has PubMed identifier	2824684
schema:publication	J Med Virol
resource representing a document's body	covid:650facc5fdb5d1fd898d60bba49ea3c06e2a5bd8#body_text
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