About: Label‐free detection techniques for protein microarrays: Prospects, merits and challenges

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Label‐free detection techniques for protein microarrays: Prospects, merits and challenges
Creator	Ray, Sandipan Srivastava, Sanjeeva Mehta, Gunjan
Source	PMC
abstract	Protein microarrays, on which thousands of discrete proteins are printed, provide a valuable platform for functional analysis of the proteome. They have been widely used for biomarker discovery and to study protein–protein interactions. The accomplishments of DNA microarray technology, which had enabled massive parallel studies of gene expression, sparked great interest for the development of protein microarrays to achieve similar success at the protein level. Protein microarray detection techniques are often classified as being label‐based and label‐free. Most of the microarray applications have employed labelled detection such as fluorescent, chemiluminescent and radioactive labelling. These labelling strategies have synthetic challenges, multiple label issues and may exhibit interference with the binding site. Therefore, development of sensitive, reliable, high‐throughput, label‐free detection techniques are now attracting significant attention. Label‐free detection techniques monitor biomolecular interactions and simplify the bioassays by eliminating the need for secondary reactants. Moreover, they provide quantitative information for the binding kinetics. In this article, we will review several label‐free techniques, which offer promising applications for the protein microarrays, and discuss their prospects, merits and challenges.
has issue date	2009-12-01(xsd:dateTime)
bibo:doi	10.1002/pmic.200900458
bibo:pmid	19953541
has license	no-cc
sha1sum (hex)	63ce0f8999f5d036a7963656aba7083fc50f8a98
schema:url	https://doi.org/10.1002/pmic.200900458
resource representing a document's title	Label‐free detection techniques for protein microarrays: Prospects, merits and challenges
has PubMed Central identifier	PMC7167936
has PubMed identifier	19953541
schema:publication	Proteomics
resource representing a document's body	covid:63ce0f8999f5d036a7963656aba7083fc50f8a98#body_text
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