About: The Nsp12-coding region of type 2 PRRSV is required for viral subgenomic mRNA synthesis

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About: The Nsp12-coding region of type 2 PRRSV is required for viral subgenomic mRNA synthesis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Nsp12-coding region of type 2 PRRSV is required for viral subgenomic mRNA synthesis
Creator	Cong, Feng Tian, Zhi-Jun Cai, Xue-Hui Tang, Yan-Dong Fang, Qiong-Qiong Liu, Yong-Gang Wang, Hai-Ming Wang, Tong-Yun Zhang, Hong-Liang
Source	PMC
abstract	As one of many nonstructural proteins of porcine reproductive and respiratory syndrome virus (PRRSV), nonstructural protein 12 (Nsp12) has received relatively little attention, and its role in virus replication, if any, is essentially unknown. By the application of reverse genetic manipulation of an infectious PRRSV clone, the current study is the first to demonstrate that Nsp12 is a key component of PRRSV replication. In addition, the biochemical properties of Nsp12 were evaluated, revealing that Nsp12 forms dimers when exposed to oxidative conditions. Furthermore, we systemically analyzed the function of Nsp12 in PRRSV RNA synthesis using a strand-specific PCR method. To our surprise, Nsp12 was not found to be involved in minus-strand genomic RNA (-gRNA) synthesis; importantly, our results indicate that Nsp12 is involved in the synthesis of both plus- and minus-strand subgenomic mRNAs (+sgmRNA and -sgmRNA). Finally, we found that the combination of cysteine 35 and cysteine 79 in Nsp12 is required for sgmRNA synthesis. To our knowledge, we are the first to report the biological role of Nsp12 in the PRRSV lifecycle, and we conclude that Nsp12 is involved in the synthesis of both + sgRNA and -sgRNA.
has issue date	2019-10-21(xsd:dateTime)
bibo:doi	10.1080/22221751.2019.1679010
bibo:pmid	31631782
has license	cc-by
sha1sum (hex)	61baa9b1ae842b28e3e7aa90a3e8034d96ba9aa9
schema:url	https://doi.org/10.1080/22221751.2019.1679010
resource representing a document's title	The Nsp12-coding region of type 2 PRRSV is required for viral subgenomic mRNA synthesis
has PubMed Central identifier	PMC6818116
has PubMed identifier	31631782
schema:publication	Emerg Microbes Infect
resource representing a document's body	covid:61baa9b1ae842b28e3e7aa90a3e8034d96ba9aa9#body_text
is schema:about of	named entity 'systemically' named entity 'genomic' named entity 'subgenomic mRNA' named entity 'RNA' named entity 'SGRNA' named entity 'PROTEIN ' named entity 'ROLE' named entity 'INFECTIOUS' named entity 'FOUND' named entity 'SYNTHESIS' named entity 'MINUS' named entity 'ATTENTION' named entity 'REPORT' named entity 'CURRENT' named entity 'REVERSE' named entity 'DIMERS' named entity 'APPLICATION' named entity 'KEY' named entity 'RNA SYNTHESIS' named entity 'MRNAS' named entity 'BIOCHEMICAL' named entity 'LITTLE' named entity 'SURPRISE' named entity 'CLONE' named entity 'OUR' named entity 'PROPERTIES' named entity 'NOT FOUND' named entity 'ONE OF' named entity 'ANALYZED' named entity 'CYSTEINE' named entity 'GENETIC MANIPULATION' named entity 'STUDY' named entity 'RESULTS' named entity 'IS A' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'FUNCTION' named entity 'EXPOSED TO' named entity 'GENOMIC' named entity 'RECEIVED' named entity 'EVALUATED' named entity 'OXIDATIVE' named entity 'REQUIRED' named entity 'KNOWLEDGE' named entity 'PRRSV' named entity 'VIRAL' named entity 'REQUIRED' named entity 'COMPONENT' named entity 'CONDITIONS' named entity 'NONSTRUCTURAL PROTEINS' named entity 'REPLICATION' named entity 'FORMS' named entity 'ADDITION' named entity 'UNKNOWN' named entity 'COMBINATION' named entity 'REVEALING' named entity 'ITS' named entity 'GRNA' named entity 'TYPE 2' named entity 'CODING REGION' named entity 'BIOLOGICAL ROLE' named entity 'MRNA SYNTHESIS' named entity 'STRAND' named entity 'USING' named entity 'VIRUS REPLICATION' named entity 'CONCLUDE' named entity 'INVOLVED' named entity 'SPECIFIC' covid:arg/61baa9b1ae842b28e3e7aa90a3e8034d96ba9aa9 named entity 'clone' named entity 'biochemical' named entity 'received' named entity 'subgenomic' named entity 'minus-strand'

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