About: Human bocavirus (HBoV) in children with respiratory tract infection by enzyme linked immunosorbent assay (ELISA) and qualitative polymerase chain reaction (PCR)

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About: Human bocavirus (HBoV) in children with respiratory tract infection by enzyme linked immunosorbent assay (ELISA) and qualitative polymerase chain reaction (PCR) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Human bocavirus (HBoV) in children with respiratory tract infection by enzyme linked immunosorbent assay (ELISA) and qualitative polymerase chain reaction (PCR)
Creator	Zaghloul, Mona
Source	Medline; PMC
abstract	BACKGROUND: Human bocavirus (HBoV) is a recently discovered parvovirus associated with mild to severe lower respiratory tract infections in children, the aim of the work was determination of human bocavirus in nasopharyngeal aspirate (NPA) of infants by qualitative PCR and determination of acute human bocavirus infection by estimation of immunoglobulin M (IgM) antibodies in serum by enzyme linked immunosorbent assay. RESULTS: Twenty two (22%) out of the 100 NPA specimens of the patients with respiratory manifestations were positive for HBoV by qualitative PCR, while ELISA revealed positive HBoV IgM antibodies in 18 (18%) patients who were also positive by PCR. Non of the controls were positive by both techniques. The correlation study between ELISA and PCR revealed high significant association, (p < 0.001, X(2 )= 36 and agreement = 96%). Also PCR detected 4 (18.1%) NPA samples as HBoV positive cases among the patients that were not identified by ELISA. This could be due to high sensitivity and efficacy of PCR. ELISA being less sensitive than RT-PCR, sensitivity was (81.8% vs 100%), the efficacy was 97.7% in ELISA versus 99.7% for RT-PCR. CONCLUSION: HBoV infections could be diagnosed in NPA of children by conventional PCR as a rapid and sensitive technique. While ELISA was a reliable serologic analysis for diagnosis of acute HBoV infection by estimation IgM antibodies in serum.
has issue date	2011-05-19(xsd:dateTime)
bibo:doi	10.1186/1743-422x-8-239
bibo:pmid	21595869
has license	cc-by
sha1sum (hex)	5ffaf1c1d5f763636ec3334c1b1547d4a8b8abb9
schema:url	https://doi.org/10.1186/1743-422x-8-239
resource representing a document's title	Human bocavirus (HBoV) in children with respiratory tract infection by enzyme linked immunosorbent assay (ELISA) and qualitative polymerase chain reaction (PCR)
has PubMed Central identifier	PMC3121704
has PubMed identifier	21595869
schema:publication	Virol J
resource representing a document's body	covid:5ffaf1c1d5f763636ec3334c1b1547d4a8b8abb9#body_text
is schema:about of	named entity 'QUALITATIVE' named entity 'RESPIRATORY TRACT INFECTION' named entity 'infection' named entity 'cases' named entity 'Human bocavirus' named entity 'serum' named entity 'antibodies' named entity 'manifestations' named entity 'CONTROLS' named entity 'IGM' named entity 'SENSITIVITY' named entity 'DUE TO' named entity 'IS A' named entity 'INFECTIONS' named entity 'IDENTIFIED BY' named entity 'RESPIRATORY' named entity 'PARVOVIRUS' named entity 'BACKGROUND' named entity 'AIM' named entity 'NASOPHARYNGEAL ASPIRATE' named entity 'RECENTLY' named entity 'TWENTY TWO' named entity 'RESULTS' named entity 'BEING' named entity 'DETERMINATION' named entity 'REVEALED' named entity 'ESTIMATION' named entity 'RAPID' named entity 'FOR DIAGNOSIS' named entity 'AGREEMENT' named entity 'INFANTS' named entity 'ASSOCIATED WITH' named entity 'NOT IDENTIFIED' named entity 'COULD BE' named entity '100%' named entity 'CORRELATION STUDY' named entity 'EFFICACY' named entity 'DETECTED' named entity 'TECHNIQUE' named entity 'IMMUNOGLOBULIN M' named entity 'PATIENTS' named entity 'MILD' named entity 'SERUM' named entity 'SIGNIFICANT' named entity 'NPA' named entity 'WORK' named entity 'CASES' named entity 'SPECIMENS' named entity 'INFECTION' named entity 'SENSITIVE' named entity 'ELISA' named entity 'SAMPLES' covid:arg/5ffaf1c1d5f763636ec3334c1b1547d4a8b8abb9 named entity 'HUMAN BOCAVIRUS' named entity 'ENZYME LINKED IMMUNOSORBENT ASSAY' named entity 'CHILDREN' named entity 'POLYMERASE CHAIN REACTION' named entity '2818' named entity 'ANTIBODIES' named entity 'ENZYME LINKED IMMUNOSORBENT ASSAY' named entity 'MANIFESTATIONS' named entity 'RELIABLE' named entity 'TECHNIQUES' named entity '28P' named entity '0.001' named entity 'LOWER RESPIRATORY TRACT INFECTIONS' named entity 'SEROLOGIC' named entity 'PCR' named entity 'CHILDREN' named entity 'WAS A' named entity 'HIGH' named entity 'HUMAN BOCAVIRUS' named entity 'SEVERE' named entity 'ASSOCIATION' named entity 'ACUTE' named entity 'ANALYSIS' named entity 'DIAGNOSED' named entity 'POSITIVE FOR' named entity '100' named entity 'RT-PCR' named entity 'HIGH SENSITIVITY'

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