About: Immunoblot studies in the differential diagnosis of porcine brucellosis: an immunodominant 62 kDa protein is related to the mycobacterial 65 kDa heat shock protein (HSP-65)

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About: Immunoblot studies in the differential diagnosis of porcine brucellosis: an immunodominant 62 kDa protein is related to the mycobacterial 65 kDa heat shock protein (HSP-65) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Immunoblot studies in the differential diagnosis of porcine brucellosis: an immunodominant 62 kDa protein is related to the mycobacterial 65 kDa heat shock protein (HSP-65)
Creator	Broughton, E Hamid, S Spencer A', S Young, D
Source	PMC
abstract	Smooth Brucella spp. share certain lipopolysaccharide antigens with other bacteria, resulting in serological cross-reactions which can prevent the definitive diagnosis of brucellosis. To identify other antigens with serodiagnostic potential, immunoblot studies following sodium dodecyl sulphate - polyacrylamide gel electrophoresis were carried out. Sera from pigs experimentally infected with Brucella suis and naturally infected feral pigs, sera from pigs from a farm with a known history of Yersinia enterocolitica 0:9 infection, Brucella Complement Fixation Test (CFT) reactor pigs (aetiology unknown) and pigs from consistently Brucella CFT negative farms were examined. Although B. suis infected pigs recognized a total of nine B. melitensis antigens, individual pigs rarely recognized more than three antigens in the range. A 62 kDa antigen was recognized by the majority (73%) of the Brucella infected pigs, but only by 10 to 23% of pigs from the other groups. This antigen was shown to be the Brucella homologue of the ubiquitous 65 kDa heat shock protein (HSP-65) family by immunoblot studies with 14 monoclonal antibodies to the Mycobacterium leprae HSP-65. Only four of these monoclones (Y1.2, ML-30, D7C and IIIC8) identified the B. melitensis 62 kDa protein suggesting that unshared, potentially Brucella specific, regions exist. Sera from Y. enterocolitica 0:9 infected pigs, CFT reactor pigs (aetiology unknown), CFT negative pigs and hyperimmune pig serum raised to Y. enterocolitica 0:9 also recognized B. melitensis antigens, most notably a 17 kDa protein. This antigen appears to be a common cross-reactive protein.
has issue date	2002-11-13(xsd:dateTime)
bibo:doi	10.1016/0378-1135(94)90085-x
bibo:pmid	8203127
has license	no-cc
sha1sum (hex)	5acb4a64dd7a05fef7a6a3bed1609f2301d949d0
schema:url	https://doi.org/10.1016/0378-1135%2894%2990085-x
resource representing a document's title	Immunoblot studies in the differential diagnosis of porcine brucellosis: an immunodominant 62 kDa protein is related to the mycobacterial 65 kDa heat shock protein (HSP-65)
has PubMed Central identifier	PMC7117492
has PubMed identifier	8203127
schema:publication	Vet Microbiol
resource representing a document's body	covid:5acb4a64dd7a05fef7a6a3bed1609f2301d949d0#body_text
is schema:about of	named entity 'reactor' named entity 'Yersinia' named entity 'aetiology' named entity 'recognized' named entity 'recognized' named entity 'Sera' named entity 'differential diagnosis' named entity 'MYCOBACTERIAL' named entity 'KDA' named entity '1.2' named entity 'MAJORITY' named entity 'CFT' named entity 'BUT' named entity 'HSP-65' named entity 'STUDIES' named entity '30%' named entity 'HOMOLOGUE' named entity 'DODECYL' named entity 'GROUPS' named entity 'RARELY' named entity 'REGIONS' named entity 'FERAL' named entity 'DEFINITIVE DIAGNOSIS' named entity 'FARM' named entity 'EXIST' named entity 'NEGATIVE' named entity 'TO IDENTIFY' named entity 'MYCOBACTERIUM LEPRAE' named entity 'EXAMINED' named entity 'CARRIED' named entity 'YERSINIA ENTEROCOLITICA' named entity 'SMOOTH' named entity 'MORE THAN' named entity 'INFECTED' named entity 'TOTAL' named entity 'KNOWN' named entity 'UNKNOWN' named entity 'INFECTION' named entity 'PIGS' named entity 'MONOCLONAL ANTIBODIES' named entity 'IMMUNOBLOT' named entity 'PROTEIN ' named entity 'STUDIES' named entity 'HEAT SHOCK PROTEIN' named entity 'SHARE' named entity 'SEROLOGICAL' named entity 'FARMS' named entity 'RESULTING IN' named entity 'POTENTIAL' named entity 'BRUCELLA' named entity 'PORCINE BRUCELLOSIS' named entity 'RELATED' named entity 'DIFFERENTIAL DIAGNOSIS' named entity 'REACTIONS' named entity 'FAMILY' named entity 'ANTIGEN' named entity 'THESE' named entity 'BRUCELLOSIS' named entity 'GEL ELECTROPHORESIS' named entity 'SODIUM' named entity 'ANTIGENS' named entity 'HISTORY OF' named entity 'BRUCELLA SUIS' named entity 'PROTEIN ' named entity 'CROSS' named entity 'REACTOR' named entity 'BACTERIA' named entity 'CERTAIN' named entity 'FOLLOWING' named entity 'SPECIFIC' named entity 'RANGE' named entity 'HEAT SHOCK PROTEIN' named entity 'LIPOPOLYSACCHARIDE' named entity 'SPP' named entity 'BRUCELLA COMPLEMENT FIXATION TEST' named entity 'SERA' named entity 'PREVENT' named entity 'IDENTIFIED'

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