About: Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs

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About: Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs
Creator	Wren, Brendan Brockmeier, Susan Loving, Crystal Nicholson, Tracy Peters, Sarah Maskell, Duncan Tucker, Alexander Weinert, Lucy Rycroft, Andrew Wang, Jinhong Hau, Samantha Langford, Paul Luan, Shi-Lu Seilly, David
Source	PMC
abstract	BACKGROUND: Glaesserella parasuis, the causative agent of Glӓsser’s disease, is widespread in swine globally resulting in significant economic losses to the swine industry. Prevention of Glӓsser’s disease in pigs has been plagued with an inability to design broadly protective vaccines, as many bacterin based platforms generate serovar or strain specific immunity. Subunit vaccines are of interest to provide protective immunity to multiple strains of G. parasuis. Selected proteins for subunit vaccination should be widespread, highly conserved, and surface exposed. RESULTS: Two candidate proteins for subunit vaccination (RlpB and VacJ) against G. parasuis were identified using random mutagenesis and an in vitro organ culture system. Pigs were vaccinated with recombinant RlpB and VacJ, outer membrane proteins with important contributions to cellular function and viability. Though high antibody titers to the recombinant proteins and increased interferon-γ producing cells were found in subunit vaccinated animals, the pigs were not protected from developing systemic disease. CONCLUSIONS: It appears there may be insufficient RlpB and VacJ exposed on the bacterial surface for antibody to bind, preventing high RlpB and VacJ specific antibody titers from protecting animals from G. parasuis. Additionally, this work confirms the importance of utilizing the natural host species when assessing the efficacy of vaccine candidates.
has issue date	2020-05-27(xsd:dateTime)
bibo:doi	10.1186/s12917-020-02377-5
has license	cc-by
sha1sum (hex)	597a9eda59c4d2cb8f37391cc54bd98751f2ad25
schema:url	https://doi.org/10.1186/s12917-020-02377-5
resource representing a document's title	Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs
has PubMed Central identifier	PMC7252510
schema:publication	BMC Vet Res
resource representing a document's body	covid:597a9eda59c4d2cb8f37391cc54bd98751f2ad25#body_text
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