About: Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China
Creator	Yuan, Jing Li, Huan Liu, Wei Bai, Changqing Guo, Leijing Lei, Hong Li, Puyuan Liu, Huiying Niu, Wenkai Yuan, Xin Zhao, Xiangna Zou, Dayang Li, P Tiwari, Vishvanath Gonçalves, Alexandre Landini, Paolo Li, Niu Samuelsen, Ørjan
source	Medline; PMC
abstract	Acinetobacter baumannii is an important opportunistic pathogen associated with a variety of nosocomial infections. A rapid and sensitive molecular detection in clinical isolates is quite needed for the appropriate therapy and outbreak control of A. baumannii. Group 2 carbapenems have been considered the agents of choice for the treatment of multiple drug-resistant A. baumannii. But the prevalence of carbapenem-resistant A. baumannii (CRAB) has been steadily increasing in recent years. Here, we developed a loop-mediated isothermal amplification (LAMP) assay for the rapid detection of A. baumannii in clinical samples by using high-specificity primers of the bla(OXA-51) gene. Then we investigated the OXA-carbapenemases molecular epidemiology of A. baumannii isolates in two comprehensive hospitals in Beijing. The results showed that the LAMP assay could detect target DNA within 60 min at 65°C. The detection limit was 50 pg/μl, which was about 10-fold greater than that of PCR. Furthermore, this method could distinguish A. baumannii from the homologous A. nosocomialis and A. pittii. A total of 228 positive isolates were identified by this LAMP-based method for A. baumannii from 335 intensive care unit patients with clinically suspected multi-resistant infections in two hospitals in Beijing. The rates of CRAB are on the rise and are slowly becoming a routine phenotype for A. baumannii. Among the CRABs, 92.3% harbored both the bla(OXA-23) and bla(OXA-51) genes. Thirty-three pulsotypes were identified by pulsed-field gel electrophoresis, and the majority belonged to clone C. In conclusion, the LAMP method developed for detecting A. baumannii was faster and simpler than conventional PCR and has great potential for both point-of-care testing and basic research. We further demonstrated a high distribution of class D carbapenemase-encoding genes, mainly OXA-23, which presents an emerging threat in hospitals in China.
has issue date	2015-09-23(xsd:dateTime)
bibo:doi	10.3389/fmicb.2015.00997
bibo:pmid	26441924
has license	cc-by
sha1sum (hex)	595582a254c921f28d7c5cb9cb42e1dcabff7108
schema:url	https://doi.org/10.3389/fmicb.2015.00997
resource representing a document's title	Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China
has PubMed Central identifier	PMC4585070
has PubMed identifier	26441924
schema:publication	Front Microbiol
resource representing a document's body	covid:595582a254c921f28d7c5cb9cb42e1dcabff7108#body_text
is schema:about of	named entity 'DETECTION' named entity 'The' named entity 'clone' named entity 'therapy' named entity 'COMPREHENSIVE' named entity 'CHINA' named entity 'RISE' named entity '80%' covid:arg/595582a254c921f28d7c5cb9cb42e1dcabff7108 named entity 'DETECT' named entity 'MOLECULAR EPIDEMIOLOGY' named entity 'ENCODING' named entity 'BLA' named entity 'MIN' named entity 'CLINICAL' named entity 'CARBAPENEMS' named entity 'MULTI' named entity 'PULSED-FIELD GEL ELECTROPHORESIS' named entity 'DRUG' named entity 'MAJORITY' named entity '335' named entity 'GREAT' named entity 'HAVE' named entity 'CRABS' named entity 'GENES' named entity 'DEMONSTRATED' named entity 'CONSIDERED' named entity 'SENSITIVE' named entity 'CLONE C' named entity 'GROUP 2' named entity 'ISOLATES' named entity 'CONVENTIONAL' named entity 'VARIETY' named entity 'SLOWLY' named entity 'PREVALENCE' named entity 'PHENOTYPE' named entity 'ACINETOBACTER BAUMANNII' named entity 'HOSPITALS' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'RESULTS' named entity 'CONCLUSION' named entity 'RESISTANT' named entity 'BEIJING' named entity 'TOTAL' named entity 'NOSOCOMIAL INFECTIONS' named entity 'SAMPLES' named entity 'CARBAPENEMASE' named entity 'TESTING' named entity 'METHOD' named entity 'RESISTANT' named entity 'THERAPY' named entity 'IDENTIFIED BY' named entity 'POSITIVE' named entity 'CRAB' named entity 'THIRTY' named entity 'SUSPECTED' named entity 'CHOICE' named entity 'PRIMERS' named entity 'HIGH DISTRIBUTION' named entity 'PRESENTS' named entity 'ASSOCIATED WITH' named entity 'HOSPITALS' named entity 'INCREASING' named entity 'FOLD'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software