About: Measurement of neutralizing antibody responses against H5N1 clades in immunized mice and ferrets using pseudotypes expressing influenza hemagglutinin and neuraminidase

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Measurement of neutralizing antibody responses against H5N1 clades in immunized mice and ferrets using pseudotypes expressing influenza hemagglutinin and neuraminidase Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Measurement of neutralizing antibody responses against H5N1 clades in immunized mice and ferrets using pseudotypes expressing influenza hemagglutinin and neuraminidase
Creator	Zhang, Guoliang Buchy, Philippe Deubel, Vincent Lu, Shan Wang, Shixia Hu, Hongxing Tsai, Cheguo Zhou, Boping Zhou, Fan Zhou, Paul Caillet, Catherine Ding, Heng Vogel, Frederick
Source	Elsevier; Medline; PMC
abstract	Abstract Neutralizing antibody is associated with the prevention and clearance of influenza virus infection. Microneutralization (MN) and hemagglutination inhibition (HI) assays are currently used to evaluate neutralizing antibody responses against human and avian influenza viruses, including H5N1. The MN assay is somewhat labor intensive, while HI is a surrogate for neutralization. Moreover, use of replication competent viruses in these assays requires biosafety level 3 (BSL-3) containment. Therefore, a neutralization assay that does not require BSL-3 facilities would be advantageous. Toward this goal, we generated a panel of pseudotypes expressing influenza hemagglutinin (HA) and neuraminidase (NA) and developed a pseudotype-based neutralization (PN) assay. Here we demonstrate that HA/NA pseudotypes mimic release and entry of influenza virus and that the PN assay exhibits good specificity and reveals quantitative difference in neutralizing antibody titers against different H5N1 clades and subclades. Using immune ferret sera, we demonstrated excellent correlation between the PN, MN, and HI assays. Thus, we conclude that the PN assay is a sensitive and quantifiable method to measure neutralizing antibodies against diverse clades and subclades of H5N1 influenza virus.
has issue date	2009-11-12(xsd:dateTime)
bibo:doi	10.1016/j.vaccine.2009.08.056
bibo:pmid	19732860
has license	els-covid
sha1sum (hex)	5325a03610cff1a81555fb64124ea2d493bdcf36
schema:url	https://doi.org/10.1016/j.vaccine.2009.08.056
resource representing a document's title	Measurement of neutralizing antibody responses against H5N1 clades in immunized mice and ferrets using pseudotypes expressing influenza hemagglutinin and neuraminidase
has PubMed Central identifier	PMC7115403
has PubMed identifier	19732860
schema:publication	Vaccine
resource representing a document's body	covid:5325a03610cff1a81555fb64124ea2d493bdcf36#body_text
is schema:about of	named entity 'MICE' named entity 'IMMUNIZED' named entity 'RESPONSES' named entity 'ENTRY' named entity 'CORRELATION' named entity 'INFLUENZA VIRUSES' named entity 'REVEALS' named entity 'IMMUNE' named entity 'PREVENTION' named entity 'ASSAY' named entity 'RELEASE' named entity 'IS A' named entity 'EXCELLENT' named entity 'REPLICATION COMPETENT VIRUSES' named entity 'NEUTRALIZATION' named entity 'DEMONSTRATED' named entity 'ASSOCIATED WITH' named entity 'GOOD' named entity 'FACILITIES' named entity 'HERE' named entity 'CONTAINMENT' named entity 'METHOD' named entity 'FERRET' named entity 'FERRETS' named entity 'DOES NOT' named entity 'QUANTIFIABLE' named entity 'BIOSAFETY LEVEL 3' named entity 'QUANTITATIVE' named entity 'NEURAMINIDASE' named entity 'NEUTRALIZING ANTIBODY' named entity 'MEASUREMENT' named entity 'USING' named entity 'TO MEASURE' named entity 'AVIAN INFLUENZA' named entity 'MIMIC' named entity 'BSL' named entity 'INFLUENZA HEMAGGLUTININ' named entity 'H5N1' named entity 'ANTIBODY TITERS' named entity 'DIVERSE' named entity 'NEURAMINIDASE' named entity 'THESE' named entity 'GOAL' named entity 'HUMAN' named entity 'PANEL' named entity 'LABOR' named entity 'EXHIBITS' named entity 'SOMEWHAT' named entity 'INFLUENZA VIRUS' named entity 'H5N1' named entity 'INFLUENZA HEMAGGLUTININ' named entity 'NEUTRALIZING ANTIBODIES' named entity 'DIFFERENCE' named entity 'SENSITIVE' named entity 'NEUTRALIZING ANTIBODY' named entity 'SERA' named entity 'H5N1 INFLUENZA' named entity 'SPECIFICITY' named entity 'CLEARANCE' named entity 'DIFFERENT' named entity 'CONCLUDE' named entity 'EVALUATE' named entity 'GENERATED' named entity 'INCLUDING' named entity 'CURRENTLY' named entity 'USED' named entity 'BASED' named entity 'HEMAGGLUTINATION INHIBITION' named entity 'USE OF'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software