About: Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes

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About: Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes
Creator	Storgaard, T Bùtner, A Madsen, K Oleksiewicz, M
Source	Elsevier; Medline; PMC
abstract	Following the recent use of a live vaccine against porcine reproductive and respiratory syndrome virus (PRRSV) in Denmark, both American (vaccine) and European-type PRRSV now coexist in Danish herds. This situation highlighted a requirement for supplementary tests for precise virus-typing. As a result, we developed a RT-PCR assay able to detect as well as type PRRSV. To provide maximal sequence information, complete viral open reading frames (ORFs 5 and 7) were targeted for amplification. The RT-PCR test was able to amplify complete PRRSV ORFs from complex materials such as boar semen containing as little as 1 TCID(50) ml(−1) of PRRSV. Typing of viruses was accomplished by any one of three strategies: (i) use of type-specific PCR primers, (ii) size determination of ORF 7 amplicons, (iii) DNA sequencing. All three typing strategies showed complete concordance with the currently used method of typing with monoclonal antibodies (MAbs) when used on a panel of PRRSV field isolates covering the period 1992–1997. The ORF 7-based test had particularly desirable characteristics, namely, highly sensitive detection of PRRSV without apparent type bias, typing of the detected virus, discrimination between pure and mixed virus populations, and semi-quantitative assessment of type ratios in mixed populations, all in a single PCR reaction. In addition, the obtained sequence data were used to predict two simple and rapid strategies (single-enzyme restriction length polymorphy analysis and oligonucleotide hybridization) for confirmation of the specificity of ORF 7 RT-PCR reactions. As such, the RT-PCR assay provides a new, powerful diagnostic tool to study the population dynamics between present and emerging PRRSV-types.
has issue date	1998-11-01(xsd:dateTime)
bibo:doi	10.1016/s0378-1135(98)00254-5
bibo:pmid	9874099
has license	no-cc
sha1sum (hex)	52754613ccb7db8607128731e0616a318e5dd92d
schema:url	https://doi.org/10.1016/s0378-1135%2898%2900254-5
resource representing a document's title	Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes
has PubMed Central identifier	PMC7117142
has PubMed identifier	9874099
schema:publication	Vet Microbiol
resource representing a document's body	covid:52754613ccb7db8607128731e0616a318e5dd92d#body_text
is schema:about of	named entity 'RT-PCR' named entity 'ORF' named entity 'determination' named entity 'detected' named entity 'maximal' named entity 'result' named entity 'herds' named entity 'isolates' named entity 'emerging' named entity 'METHOD' named entity 'DIAGNOSTIC TOOL' named entity 'PREDICT' named entity 'ONE OF' named entity 'SEMI-QUANTITATIVE' named entity 'PERIOD' named entity 'SEQUENCE' named entity 'RT-PCR ASSAY' named entity 'NEW' named entity 'OBTAINED' named entity 'PRECISE' named entity 'RESULT' named entity 'DANISH' named entity '1135' named entity 'SEMEN' covid:arg/52754613ccb7db8607128731e0616a318e5dd92d named entity 'AMERICAN' named entity 'BIAS' named entity 'ADDITION' named entity 'DETECTED' named entity 'USE OF' named entity 'SCIENCE' named entity 'MATTER' named entity 'ENZYME' named entity 'CONTAINING' named entity 'DENMARK' named entity 'STRATEGIES' named entity '2 5' named entity 'POPULATIONS' named entity 'DETECTION' named entity 'PURE' named entity 'OPEN READING FRAMES' named entity 'RAPID' named entity 'VIRAL' named entity 'TO DETECT' named entity 'CONCORDANCE' named entity 'HIGHLY SENSITIVE' named entity 'APPARENT' named entity 'COMPLEX' named entity 'FIELD' named entity 'POPULATION DYNAMICS' named entity 'MAXIMAL' named entity 'TESTS' named entity 'ORF' named entity 'VIRUSES' named entity 'HYBRIDIZATION' named entity 'CURRENTLY' named entity 'SIMPLE' named entity 'SIZE' named entity 'PANEL' named entity 'TEST' named entity 'ANALYSIS' named entity 'DISCRIMINATION' named entity 'ASSESSMENT' named entity '3 5' named entity 'TARGETED' named entity 'RECENT' named entity 'BOAR' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'RATIOS' named entity 'TYPING' named entity 'OLIGONUCLEOTIDE' named entity 'INFORMATION' named entity '1998' named entity 'POWERFUL' named entity 'STUDY' named entity 'PCR PRIMERS' named entity 'LITTLE' named entity 'CHARACTERISTICS'

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