About: Detection of feline coronaviruses in cell cultures and in fresh and fixed feline tissues using polymerase chain reaction

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Detection of feline coronaviruses in cell cultures and in fresh and fixed feline tissues using polymerase chain reaction Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of feline coronaviruses in cell cultures and in fresh and fixed feline tissues using polymerase chain reaction
Creator	Li, Xiantang Scott, Fredric
Source	Elsevier; Medline; PMC
abstract	Abstract Feline coronavirus infections in cell cultures and in fresh and fixed feline tissues were detected using a polymerase chain reaction (PCR) test. Cell cultures were inoculated with feline infectious peritonitis virus (FIPV), feline enteric coronavirus (FECV)_or sham inoculum. The tissue samples of liver, kidney and spleen were taken from specific-pathogen-free (SPF) cats that were inoculated intranasally with 103 TCID50 of FIPV 79-1146 (n = 10), FIPV UCD1 (n = 3) or sham inoculum (n = 3), from clinical cats (n = 43), and from formalin-fixed archived feline tissues (n = 49), respectively. Additional tissue samples were taken from the FIPV-inoculated cats (n = 6) and were kept at 4°C, room temperatures (20–24°C) and 37°C respectively for 0, 6, 12, 24, 48, 72, and 96 hours before frozen (−70°C) for PCR to evaluate the effects of the ambient temperatures and post-mortem intervals on the test. The samples were also fixed in 10% neutrally buffered formalin, 95% ethanol, and Bouin's solution respectively to evaluate the effects of the fixatives on the test. Positive PCR results were obtained from the cell cultures that were inoculated with FIPV and FECV and from the FIPV-inoculated cats (13/13). Negative PCR results were obtained from the sham-inoculated cell cultures and cats (3/3). Of the 92 clinical cats, 7 of the 8 FIP-suspected cats (87.5%) and 51 of the 84 non-FIP-suspected cats (60.7%) were shown to be virus-positive in at least one of the tissue samples. There was no significant difference in the PCR results between the fresh and the formalin-fixed tissues of the clinical cats (P > 0.05). Of the FIPV inoculated cats, the virus was detectable equally well in fresh and formalin-, Bouin's solution- or ethanol-fixedtissues. However, the amounts of total RNA extracted from the fixed tissues were significantly less than those from fresh tissues (P < 0.01). In tissues that were kept at 4°C, the virus was detectable up to 96 h; at room temperatures, up to 48 h; and at 37°C, up to 24 h, respectively.
has issue date	1994-09-30(xsd:dateTime)
bibo:doi	10.1016/0378-1135(94)90078-7
bibo:pmid	7839586
has license	els-covid
sha1sum (hex)	4fab0e3c956d68e8d7fd37285e85568fd49c5847
schema:url	https://doi.org/10.1016/0378-1135%2894%2990078-7
resource representing a document's title	Detection of feline coronaviruses in cell cultures and in fresh and fixed feline tissues using polymerase chain reaction
has PubMed Central identifier	PMC7117364
has PubMed identifier	7839586
schema:publication	Veterinary Microbiology
resource representing a document's body	covid:4fab0e3c956d68e8d7fd37285e85568fd49c5847#body_text
is schema:about of	named entity 'BUFFERED FORMALIN' named entity 'SAMPLES' named entity 'ADDITIONAL' named entity '1146' named entity 'ROOM' named entity 'SHAM' named entity 'CLINICAL' named entity 'TEST' named entity 'SPECIFIC' named entity 'FIPV' named entity 'cats' named entity 'cell cultures' named entity 'inoculated' named entity 'ethanol' named entity 'tissues' named entity 'intervals' named entity 'ambient temperatures' named entity 'DETECTION' named entity 'INTERVALS' named entity '370' named entity 'FELINE INFECTIOUS PERITONITIS VIRUS' named entity 'USING' named entity 'CORONAVIRUSES' named entity 'FRESH' named entity 'TISSUES' named entity 'CORONAVIRUS INFECTIONS' named entity 'FREE' named entity 'FELINE' named entity 'TISSUE' named entity 'HOURS' named entity 'SPLEEN' named entity '10%' named entity '3D3' named entity 'EVALUATE' named entity 'CATS' named entity '103' named entity '282' named entity 'LIVER' named entity 'CELL CULTURES' named entity 'POLYMERASE CHAIN REACTION' named entity 'FIXED' named entity 'FRESH' named entity 'FIXATIVES' named entity 'FROZEN' named entity 'USING' named entity 'DETECTED' named entity 'INOCULUM' named entity 'FIXED' named entity 'KIDNEY' named entity 'FELINE CORONAVIRUS' named entity 'SOLUTION' named entity 'TEMPERATURES' named entity 'EFFECTS' named entity 'FELINE' named entity 'FORMALIN' named entity 'ARCHIVED' named entity 'TAKEN' named entity '27S' named entity 'POST-MORTEM' named entity '70%' named entity 'POLYMERASE CHAIN REACTION' named entity 'CELL CULTURES' named entity 'TISSUES' named entity 'PATHOGEN' named entity 'AMBIENT' named entity 'ETHANOL' named entity 'PCR' covid:arg/4fab0e3c956d68e8d7fd37285e85568fd49c5847 named entity 'infections' named entity 'enteric' named entity 'feline' named entity 'tissues' named entity 'formalin' named entity 'fresh' named entity 'chain reaction' named entity 'PCR' named entity 'ambient temperatures' named entity 'Cell cultures' named entity 'kidney' named entity 'liver'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software