About: In vivo Biodistribution of a Highly Attenuated Recombinant Vesicular Stomatitis Virus Expressing HIV-1 Gag Following Intramuscular, Intranasal, or Intravenous Inoculation

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About: In vivo Biodistribution of a Highly Attenuated Recombinant Vesicular Stomatitis Virus Expressing HIV-1 Gag Following Intramuscular, Intranasal, or Intravenous Inoculation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	In vivo Biodistribution of a Highly Attenuated Recombinant Vesicular Stomatitis Virus Expressing HIV-1 Gag Following Intramuscular, Intranasal, or Intravenous Inoculation
Creator	Calderon, Priscilla Cooper, David Obregon, Jennifer Ogin-Wilson, Eleanor Clarke, David Kalyan, Narender Coleman, John Hendry, R Johnson, J Natuk, Robert Udem, Stephen Wright, Kevin
Source	Elsevier; Medline; PMC
abstract	Recombinant vesicular stomatitis viruses (rVSVs) are being developed as potential HIV-1 vaccine candidates. To characterize the in vivo replication and dissemination of rVSV vectors in mice, high doses of a highly attenuated vector expressing HIV-1 Gag, rVSV(IN)- N4CT9-Gag1, and a prototypic reference virus, rVSV(IN)-HIVGag5, were delivered intramuscularly (IM), intranasally (IN), or intravenously (IV). We used quantitative, real-time RT-PCR (Q-PCR) and standard plaque assays to measure the temporal dissemination of these viruses to various tissues. Following IM inoculation, both viruses were detected primarily at the injection site as well as in draining lymph nodes; neither virus induced significant weight loss, pathologic signs, or evidence of neuroinvasion. In contrast, following IN inoculation, the prototypic virus was detected in all tissues tested and caused significant weight loss leading to death. IN administration of rVSV(IN)- N4CT9-Gag1 resulted in detection in numerous tissues (brain, lung, nasal turbinates, and lymph nodes) albeit in significantly reduced levels, which caused little or no weight loss nor any mortality. Following IV inoculation, both prototypic and attenuated viruses were detected by Q-PCR in all tissues tested. In contrast to the prototype, rVSV(IN)-N4CT9-Gag1 viral loads were significantly lower in all organs tested, and no infectious virus was detected in the brain following IV inoculation, despite the presence of viral RNA. These studies demonstrated significant differences in the biodistribution patterns of and the associated pathogenicity engendered by the prototypic and attenuated vectors in a highly susceptible host.
has issue date	2009-05-01(xsd:dateTime)
bibo:doi	10.1016/j.vaccine.2009.03.006
bibo:pmid	19428903
has license	green-oa
sha1sum (hex)	4bab96d5af96bc1c85e0c95000c26e58c0c9ef0d
schema:url	https://doi.org/10.1016/j.vaccine.2009.03.006
resource representing a document's title	In vivo Biodistribution of a Highly Attenuated Recombinant Vesicular Stomatitis Virus Expressing HIV-1 Gag Following Intramuscular, Intranasal, or Intravenous Inoculation
has PubMed Central identifier	PMC2747378
has PubMed identifier	19428903
schema:publication	Vaccine
resource representing a document's body	covid:4bab96d5af96bc1c85e0c95000c26e58c0c9ef0d#body_text
is schema:about of	named entity 'inoculation' named entity 'biodistribution' named entity 'highly' named entity 'intravenously' named entity 'organs' named entity 'virus' named entity 'NO WEIGHT LOSS' named entity 'TESTED' named entity 'PCR' named entity 'VIRAL RNA' named entity 'REFERENCE' named entity 'PLAQUE' named entity 'HIGH' named entity 'LOWER' named entity 'DOSES' named entity 'LYMPH NODES' named entity 'LEADING' named entity 'VARIOUS' named entity 'VECTOR' covid:arg/4bab96d5af96bc1c85e0c95000c26e58c0c9ef0d named entity 'DEMONSTRATED' named entity 'PROTOTYPE' named entity 'EVIDENCE OF' named entity 'INJECTION SITE' named entity 'PATHOLOGIC' named entity 'DEATH' named entity 'SIGNS' named entity 'POTENTIAL' named entity 'NASAL TURBINATES' named entity 'SIGNIFICANT' named entity 'NUMEROUS' named entity 'STANDARD' named entity 'DETECTED' named entity 'LEVELS' named entity 'ASSOCIATED' named entity 'VACCINE' named entity 'INFECTIOUS' named entity 'QUANTITATIVE' named entity 'VESICULAR STOMATITIS ' named entity 'INTRAVENOUSLY' named entity 'USED' named entity 'VIRUSES' named entity 'REAL-TIME RT-PCR' named entity 'DRAINING' named entity 'WEIGHT LOSS' named entity 'ORGANS' named entity 'FOLLOWING' named entity 'ALL TISSUES' named entity 'TEMPORAL' named entity 'DELIVERED' named entity 'BIODISTRIBUTION' named entity 'SUSCEPTIBLE' named entity 'PATTERNS' named entity 'RVSV' named entity 'GAG' named entity 'IN VIVO' named entity 'RECOMBINANT' named entity 'CAUSED' named entity 'NEITHER' named entity 'VIRAL' named entity 'ADMINISTRATION' named entity 'HOST' named entity 'LITTLE' named entity 'REDUCED' named entity 'INOCULATION' named entity 'BEING' named entity 'PRESENCE OF' named entity 'STUDIES' named entity 'TO MEASURE' named entity 'CONTRAST'

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