About: Culturing of respiratory viruses in well-differentiated pseudostratified human airway epithelium as a tool to detect unknown viruses

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About: Culturing of respiratory viruses in well-differentiated pseudostratified human airway epithelium as a tool to detect unknown viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Culturing of respiratory viruses in well-differentiated pseudostratified human airway epithelium as a tool to detect unknown viruses
Creator	Dijkman, Ronald Molenkamp, Richard Deijs, Martin Goossens, Herman Ieven, Margareta Farsani, Jazaeri Jeeninga, Rienk Mohammad, Seyed Van Der Hoek, Lia
Source	Medline; PMC
abstract	BACKGROUND: Currently, virus discovery is mainly based on molecular techniques. Here, we propose a method that relies on virus culturing combined with state-of-the-art sequencing techniques. The most natural ex vivo culture system was used to enable replication of respiratory viruses. METHOD: Three respiratory clinical samples were tested on well-differentiated pseudostratified tracheobronchial human airway epithelial (HAE) cultures grown at an air–liquid interface, which resemble the airway epithelium. Cells were stained with convalescent serum of the patients to identify infected cells and apical washes were analyzed by VIDISCA-454, a next-generation sequencing virus discovery technique. RESULTS: Infected cells were observed for all three samples. Sequencing subsequently indicated that the cells were infected by either human coronavirus OC43, influenzavirus B, or influenzavirus A. The sequence reads covered a large part of the genome (52%, 82%, and 57%, respectively). CONCLUSION: We present here a new method for virus discovery that requires a virus culture on primary cells and an antibody detection. The virus in the harvest can be used to characterize the viral genome sequence and cell tropism, but also provides progeny virus to initiate experiments to fulfill the Koch's postulates.
has issue date	2014-12-04(xsd:dateTime)
bibo:doi	10.1111/irv.12297
bibo:pmid	25482367
has license	cc-by
sha1sum (hex)	4ae32ac7470a95f95ccd2309bfb62a990c1e0e16
schema:url	https://doi.org/10.1111/irv.12297
resource representing a document's title	Culturing of respiratory viruses in well-differentiated pseudostratified human airway epithelium as a tool to detect unknown viruses
has PubMed Central identifier	PMC4280819
has PubMed identifier	25482367
schema:publication	Influenza Other Respir Viruses
resource representing a document's body	covid:4ae32ac7470a95f95ccd2309bfb62a990c1e0e16#body_text
is schema:about of	named entity 'TO DETECT' named entity 'Background' named entity 'WELL-DIFFERENTIATED' named entity 'STATE' named entity 'NATURAL' named entity 'TECHNIQUES' named entity 'CULTURING' named entity 'METHOD' named entity 'REPLICATION' named entity 'HERE' named entity 'BASED' named entity 'RESPIRATORY VIRUSES' named entity 'USED' named entity 'DISCOVERY' named entity 'ENABLE' named entity 'CURRENTLY' named entity 'CULTURE' named entity 'ART' named entity 'VIRUS' named entity 'RESPIRATORY VIRUSES' named entity 'AIRWAY EPITHELIUM' named entity 'HUMAN' named entity 'PSEUDOSTRATIFIED' named entity 'TOOL' named entity 'MOLECULAR' named entity 'SYSTEM' named entity 'BACKGROUND' named entity 'OF-' named entity 'SEQUENCING TECHNIQUES' named entity 'VIRUSES' named entity 'UNKNOWN' named entity 'CULTURING' named entity 'COMBINED' named entity 'EX VIVO' named entity 'virus' named entity 'vivo' named entity 'molecular' named entity 'viruses' named entity 'viruses' named entity 'viruses' named entity 'Culturing' named entity 'high-throughput' named entity 'Invitrogen' named entity 'IgA' named entity 'IgG' named entity 'virus' named entity 'virus' named entity 'serum' named entity 'enzyme' named entity 'differentiated cells' named entity 'human coronavirus OC43' named entity 'convalescent serum' named entity 'phosphate-buffered saline' named entity 'inoculation' named entity 'Influenzavirus' named entity 'confocal microscope' named entity 'Invitrogen' named entity 'Poland' named entity 'virus' named entity 'cells differentiate' named entity 'convalescent serum' named entity 'PBS' named entity 'virus' named entity 'serology' named entity 'virus' named entity 'resection' named entity 'University of Tübingen' named entity 'convalescent serum' named entity 'H3N2' named entity 'upper airway' named entity 'intestine' named entity 'respiratory infection' named entity 'infection'

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