About: Detection of Antibodies Against Canine Circovirus in Naturally and Experimentally Infected Canines by Recombinant Capsid Enzyme-Linked Immunosorbent Assay

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About: Detection of Antibodies Against Canine Circovirus in Naturally and Experimentally Infected Canines by Recombinant Capsid Enzyme-Linked Immunosorbent Assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of Antibodies Against Canine Circovirus in Naturally and Experimentally Infected Canines by Recombinant Capsid Enzyme-Linked Immunosorbent Assay
Creator	Wang, Yu Wang, Zheng Cui, Wen Wang, Y Chen, Hongyan Ge, Junwei Cui, Xingyang Kalaiyarasu, Semmannan Rivera-Benítez, Francisco Shi, Yunjia Zhao, Lili Wen, Shanshan Cn, Chenhongyan@caas Liu, Hanghang Shi, Wang
Source	PMC
abstract	Canine circovirus (CanineCV), a new pathogen, was found to be associated with canine hemorrhagic diarrhea, vasculitis, granulomatous lymphadenitis, and acute gastroenteritis. Although CanineCV was highly positive rate in diarrhea cases, its pathogenicity remains controversial. In this study, the seroprevalence and associated risk factors of CanineCV infection among domestic dogs in northeastern China was investigated by an indirect enzyme-linked immunosorbent assay (iELISA) based on recombinant capsid protein. Results revealed the proposed iELISA had no cross-reactivity with other related pathogens, and yielded good diagnostic values. Then, to evaluate the rCap iELISA, this study applied it to detect antibodies against CanineCV in 1,047 clinical serum samples obtained from northeastern China in 2016–2017. Results showed the positive rates in the five cities of Jilin, Liaoning, and Heilongjiang provinces ranged from 22.22 to 42.29%. Statistical analysis shows a significant difference in age between dogs <3 months old with respect to the >1-year-old dogs (p = 0.005), that is, the CanineCV infection was more frequently identified from older dogs. In the artificially infected experiment, the dogs developed seroconversion after 9 or 12 days and the main way of virus excretion was through feces. More interestingly, among the 32 ELISA-positive serum samples, 34.75% samples tested positive for the CanineCV DNA by qPCR, far higher than that in ELISA-negative serum samples (5.26%, 2/38). This report is the first to demonstrate that CanineCV infection is common in the dog population in northeastern China. The results showed obvious differences in the positive rate associated with diarrhea, age, but not with different cities. This study also provide basis for evaluating the pathogenic potential of CanineCV. But, the pathogenicity, the relationship between antibody level and immune protection, and the harmful effects of this virus remain to be established.
has issue date	2020-05-28(xsd:dateTime)
bibo:doi	10.3389/fvets.2020.00294
has license	cc-by
sha1sum (hex)	49bf843edf8df1c2e9e597867b10de5a345ff0c8
schema:url	https://doi.org/10.3389/fvets.2020.00294
resource representing a document's title	Detection of Antibodies Against Canine Circovirus in Naturally and Experimentally Infected Canines by Recombinant Capsid Enzyme-Linked Immunosorbent Assay
has PubMed Central identifier	PMC7270207
schema:publication	Front Vet Sci
resource representing a document's body	covid:49bf843edf8df1c2e9e597867b10de5a345ff0c8#body_text
is schema:about of	named entity 'Capsid' named entity 'Canine Circovirus' named entity 'S. typhimurium' named entity 'incidence rates' named entity '1:25' named entity 'weight loss' named entity 'Northeast Agricultural University' named entity 'IgG' named entity 'cesarean' named entity 'serological' named entity 'signs of disease' named entity 'pathogenicity' named entity 'serum samples' named entity 'enzyme-linked immunosorbent assay' named entity 'fusion protein' named entity 'amino terminal' named entity 'respiratory distress' named entity 'virus' named entity '3SD' named entity 'IgG' named entity 'serological survey' named entity 'positive reaction' named entity 'seropositive' named entity 'recombinant protein' named entity 'China' named entity 'genetic recombination' named entity 'CAV2' named entity 'serological surveys' named entity 'control dog' named entity 'pathological conditions' named entity 'expression vector' named entity 'Canine circovirus' named entity 'cross-reactions' named entity 'statistical analysis' named entity 'lymphadenitis' named entity 'skimmed milk' named entity 'long time' named entity 'gavage' named entity 'quantitative real-time PCR' named entity 'infection' named entity 'primer' named entity 'IgG' named entity 'antibody' named entity 'electrophoresis' named entity 'kDa' named entity 'epidemiological' named entity 'epidemiology' named entity 'Circovirus' named entity 'Italy' named entity 'vector' named entity 'virus' named entity 'CDV' named entity 'serum' named entity 'real-time PCR' named entity 'Western blot' named entity 'polymerase chain reaction' named entity 'serially diluted' named entity 'recombinant E. coli' named entity 'His-tagged' named entity 'Western blot' named entity 'protein' named entity 'CDV' named entity 'PCR' named entity 'serum samples' named entity 'granulomatous' named entity 'antigen' named entity 'pET' named entity 'canine' named entity 'pET'

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