About: LIPS method for the detection of SARS‐CoV‐2 antibodies to spike and nucleocapsid proteins

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About: LIPS method for the detection of SARS‐CoV‐2 antibodies to spike and nucleocapsid proteins Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	LIPS method for the detection of SARS‐CoV‐2 antibodies to spike and nucleocapsid proteins
Creator	Haljasmägi, Liis Kisand, Kai Krassohhina, Ekaterina Peterson, Pärt Remm, Anu Rumm, Anna Sein, Hanna Tamm, Anu
Source	Medline; PMC
abstract	Profiling antibodies to SARS‐CoV‐2 can help to assess potential immune response after COVID‐19 disease. Luciferase IP system (LIPS) assay is a sensitive method for quantitative detection of antibodies to antigens in their native conformation. We here describe LIPS to detect antibody responses to SARS‐CoV‐2 spike (S) and nucleocapsid (N) proteins in COVID‐19 patients. The antibodies targeted both S and N fragments and gave a high assay sensitivity by identifying 26 out of 26 COVID‐19 patients with N antigen or with three protein fragments when combined into a single reaction. The assay correlated well with ELISA method and was specific to COVID‐19 as we saw no reactivity among uninfected healthy controls. Our results show that LIPS is a rapid and measurable method to screen antibody responses against SARS‐CoV‐2 antigens.
has issue date	2020-07-06(xsd:dateTime)
bibo:doi	10.1002/eji.202048715
bibo:pmid	32584420
has license	no-cc
sha1sum (hex)	475a83f8650e7c729066406965d0354224337bde
schema:url	https://doi.org/10.1002/eji.202048715
resource representing a document's title	LIPS method for the detection of SARS‐CoV‐2 antibodies to spike and nucleocapsid proteins
has PubMed Central identifier	PMC7361618
has PubMed identifier	32584420
schema:publication	Eur J Immunol
resource representing a document's body	covid:475a83f8650e7c729066406965d0354224337bde#body_text
is schema:about of	named entity 'SARS-CoV-2' named entity 'transfection' named entity 'SARS-CoV-2' named entity 'luminescence' named entity 'SARS-CoV-2' named entity 'antibodies' named entity 'incubation' named entity 'IgG' named entity 'protein subunit' named entity 'plasma' named entity 'PerkinElmer' named entity 'transfected' named entity '37 °C' named entity 'Promega' named entity 'antibody' named entity 'PVDF' named entity 'immune complexes' named entity 'antibody' named entity 'gene' named entity 'room temperature' named entity 'post-translational modifications' named entity 'plasma' named entity '450 nm' named entity 'Covid-19' named entity 'chromogen' named entity '1:500' named entity 'antigens' named entity 'standard deviations' named entity 'optical density' named entity 'plasma' named entity 'viral proteins' named entity 'antibody' named entity 'Protein' named entity 'luminescence' named entity 'antibody' named entity 'transfected' named entity 'microplate reader' named entity 'polyacrylamide gel' named entity 'luminescence' named entity 'kDa' named entity 'Merck Millipore' named entity 'Promega' named entity 'cell lysates' named entity 'Promega' named entity 'antigen' named entity 'lysates' named entity 'IgG' named entity 'ELISA assay' named entity 'HEK293 cells' named entity 'molecular mass' named entity 'PerkinElmer' named entity 'calibrator' named entity 'antibody' named entity 'protein' named entity 'molecular marker' named entity 'vector' named entity 'luciferase' named entity 'SARS-CoV-2' named entity 'luciferase' named entity 'protein' named entity 'cloned' named entity 'vector' named entity 'GENE' named entity 'PROTEINS' named entity 'MODIFIED' named entity 'O C' named entity 'CONTAINING' named entity 'FUSION' named entity 'HEK293 CELLS' named entity 'Assay' named entity 'antigens' named entity 'Student's t-test' named entity 'antibody complexes' named entity 'Western blot'

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