About: Utility Evaluation of Porcine Enteroids as PDCoV Infection Model in vitro

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About: Utility Evaluation of Porcine Enteroids as PDCoV Infection Model in vitro Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Utility Evaluation of Porcine Enteroids as PDCoV Infection Model in vitro
Creator	Jung, Kwonil Chen, Yunlu Fang, Weihuan Li, Xiaoliang Luo, Hao Nilubol, Dachrit Shan, Ying Adachi, Akio Baldridge, Megan Kenney, Scott Wang, Tingjun Xu, Jidong Yue, Min Zhang, Zhenning Zheng, Jingyou
Source	Medline; PMC
abstract	Porcine deltacoronavirus (PDCoV) is a novel emerging enteric coronavirus found in pigs. Intestinal enteroids, which partially recreate the structure and function of intestinal villi-crypts, have many physiological similarities to the intestinal tissues in vivo. Enteroids exhibit advantages in studying the interactions between intestines and enteric pathogens. To create a novel infection model for PDCoV, we developed an in vitro system to generate porcine intestinal enteroids from crypts of duodenum, jejunum, and ileum of pigs. Enterocytes, enteroendocrine cells, Paneth cells, stem cells, proliferating cells, and goblet cells were found in the differentiated enteroids. Replication of PDCoV was detected in the cultured enteroids by immunofluorescence and quantitative RT-PCR. Double immunofluorescence labeling demonstrated that PDCoV was present in Sox9-positive intestinal cells and Villin1-positive enterocytes. There were multiple cellular responses shown as changes of transcription of genes related to mucosal immunity, antiviral genes, and marker genes of stem cells and other cells in the enteroids infected with PDCoV. We conclude that the 2-D enteroids derived from porcine jejunum can be used as an in vitro multicellular model for the investigation of pathogenesis and host immune responses to porcine enteric pathogens, such as PDCoV.
has issue date	2020-04-23(xsd:dateTime)
bibo:doi	10.3389/fmicb.2020.00821
bibo:pmid	32390999
has license	cc-by
sha1sum (hex)	3d49b0a42f1a6fe21ffdeca12505b0ff893b36b7
schema:url	https://doi.org/10.3389/fmicb.2020.00821
resource representing a document's title	Utility Evaluation of Porcine Enteroids as PDCoV Infection Model in vitro
has PubMed Central identifier	PMC7191032
has PubMed identifier	32390999
schema:publication	Front Microbiol
resource representing a document's body	covid:3d49b0a42f1a6fe21ffdeca12505b0ff893b36b7#body_text
is schema:about of	named entity 'immunofluorescence' named entity 'emerging' named entity 'responses' named entity 'transcription' named entity 'Enterocytes' named entity 'IS A' named entity 'INVESTIGATION' named entity 'GENES' named entity 'PATHOGENS' named entity 'MULTIPLE' named entity 'QUANTITATIVE RT-PCR' named entity 'PORCINE' covid:arg/3d49b0a42f1a6fe21ffdeca12505b0ff893b36b7 named entity 'goblet cells' named entity 'RT-PCR' named entity 'Paneth cells' named entity 'jejunum' named entity 'mucosal immunity' named entity 'transcription of genes' named entity 'intestinal villi' named entity 'porcine' named entity 'intestinal cells' named entity 'Sox9' named entity 'stem cells' named entity 'quantitative RT-PCR' named entity 'proliferating cells' named entity 'Paneth cells' named entity 'coronavirus' named entity 'enteroendocrine cells' named entity 'pigs' named entity 'PEDV' named entity 'Lgr5' named entity 'deltacoronavirus' named entity 'intestinal stem cells' named entity 'Laos' named entity 'enterocytes' named entity 'intestinal crypts' named entity 'RNA' named entity 'cell marker' named entity 'viral infection' named entity 'E-cadherin' named entity 'microorganisms' named entity 'infection' named entity 'duodenum' named entity 'pathogens' named entity 'Sox9' named entity 'protein' named entity 'Nutrient' named entity 'mucosal' named entity 'pathogenesis' named entity 'enteroendocrine cells' named entity 'antibodies' named entity 'Peyer's patch' named entity 'infection' named entity 'intestinal epithelial cell' named entity 'antibacterial peptides' named entity 'gene' named entity 'ileum' named entity 'ileum' named entity 'type 2' named entity 'swine' named entity 'centrifuging' named entity 'Lyz' named entity 'Transcription' named entity 'DAPI' named entity 'monoclonal antibodies' named entity 'infection'

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