About: Detection of economically important viruses in boar semen by quantitative RealTime PCR™ technology

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of economically important viruses in boar semen by quantitative RealTime PCR™ technology
Creator	Feitsma, Hanneke Jacobs, Liesbeth Van Rijn, Piet Hakze-Van Der Honing, Renate Moonen, Peter Wellenberg, Gerard
Source	Elsevier; Medline; PMC
abstract	The objective of this study was to develop quantitative real-time polymerase chain reaction (ReTi-PCR) tests for the detection of five economically important viruses in swine semen namely, pseudorabies virus (PRV), classical swine fever virus (CSFV), foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), and porcine reproductive and respiratory syndrome virus (PRRSV). Each ReTi-PCR test was validated for specificity, analytical sensitivity (detection limits), and experimental infection studies were performed to compare the conventional virus isolation methods with the newly developed ReTi-PCR tests. All five developed ReTi-PCR tests are very rapid compared to virus isolation, highly specific, and even more sensitive (lower detection limits) than conventional virus isolation methods for the detection of mentioned viruses in semen. In semen of experimentally infected boars, viruses were detected much earlier after infection and more frequently by ReTi-PCR tests than by virus isolations. The high throughput of these rapid ReTi-PCR tests makes it possible to screen large number of semen samples for the presence of viruses prior to insemination. This is a substantial advantage, in particular for boar semen the quality of which deteriorates quickly after storage. In general, the newly developed ReTi-PCR tests are valuable tools for the early, reliable and rapid detection of five economically important viruses, namely PRV, CSFV, FMDV, SVDV, and PRRSV in boar semen. These ReTi-PCR tests will improve the control of viral diseases transmitted via semen.
has issue date	2004-09-15(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2004.04.014
bibo:pmid	15288957
has license	no-cc
sha1sum (hex)	3c29f187d1803120d1ce51c79886e6833ba0d772
schema:url	https://doi.org/10.1016/j.jviromet.2004.04.014
resource representing a document's title	Detection of economically important viruses in boar semen by quantitative RealTime PCR™ technology
has PubMed Central identifier	PMC7119495
has PubMed identifier	15288957
schema:publication	J Virol Methods
resource representing a document's body	covid:3c29f187d1803120d1ce51c79886e6833ba0d772#body_text
is schema:about of	named entity 'DETECTION' named entity 'analytical' named entity 'developed' named entity 'tests' named entity 'economically' named entity 'BOAR' named entity 'TECHNOLOGY' named entity 'SEMEN' named entity 'TESTS' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'LIMITS' named entity 'TEST' named entity 'PSEUDORABIES VIRUS' named entity 'SWINE VESICULAR DISEASE VIRUS' named entity 'SWINE' named entity 'TO COMPARE' named entity 'REALTIME' named entity 'PCR' named entity 'QUANTITATIVE' named entity 'SEMEN' named entity 'STUDIES' named entity 'ANALYTICAL' named entity 'EXPERIMENTAL INFECTION' named entity 'NEWLY' named entity 'VIRUSES' named entity 'VIRUSES' named entity 'METHODS' named entity 'SENSITIVITY' named entity 'CLASSICAL SWINE FEVER VIRUS' named entity 'DETECTION' named entity 'RETI' named entity 'ECONOMICALLY IMPORTANT' named entity 'PERFORMED' named entity 'CONVENTIONAL' named entity 'PCR' named entity 'SPECIFICITY' named entity 'ECONOMICALLY IMPORTANT' named entity 'FOOT-AND-MOUTH DISEASE VIRUS' named entity 'QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION' named entity 'VALIDATED' named entity 'VIRUS ISOLATION' named entity 'STUDY' named entity 'OBJECTIVE' covid:arg/3c29f187d1803120d1ce51c79886e6833ba0d772 named entity 'performed' named entity 'study' named entity 'porcine' named entity 'virus' named entity 'limits' named entity 'PRRSV' named entity 'PCR tests' named entity 'infection' named entity 'pseudorabies virus' named entity 'PRV' named entity 'boar' named entity 'semen' named entity 'macrophages' named entity 'semen' named entity 'semen' named entity 'semen' named entity 'FMDV' named entity 'incubation period' named entity 'semen' named entity 'virus' named entity 'semen' named entity 'virus isolation' named entity 'antigens' named entity 'FMDV' named entity 'semen' named entity 'antibody' named entity 'Serological tests' named entity 'virus isolation' named entity 'FMDV' named entity 'semen' named entity 'PRRSV' named entity 'PCR'

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