About: PEDV enters cells through clathrin-, caveolae-, and lipid raft-mediated endocytosis and traffics via the endo-/lysosome pathway

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About: PEDV enters cells through clathrin-, caveolae-, and lipid raft-mediated endocytosis and traffics via the endo-/lysosome pathway Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	PEDV enters cells through clathrin-, caveolae-, and lipid raft-mediated endocytosis and traffics via the endo-/lysosome pathway
Creator	Cao, Yongchang Xue, Chunyi She, Gaoli Wei, Xiaona Wu, Tingting
Source	Medline; PMC
abstract	With the emergence of highly pathogenic variant strains, porcine epidemic diarrhea virus (PEDV) has led to significant economic loss in the global swine industry. Many studies have described how coronaviruses enter cells, but information on PEDV invasion strategies remains insufficient. Given that the differences in gene sequences and pathogenicity between classical and mutant strains of PEDV may lead to diverse invasion mechanisms, this study focused on the cellular entry pathways and cellular transport of the PEDV GI and GII subtype strains in Vero cells and IPEC-J2 cells. We first characterized the kinetics of PEDV entry into cells and found that the highest invasion rate of PEDV was approximately 33% in the IPEC-J2 cells and approximately 100% in the Vero cells. To clarify the specific endocytic pathways, systematic research methods were used and showed that PEDV enters cells via the clathrin- and caveolae-mediated endocytosis pathways, in which dynamin II, clathrin heavy chain, Eps15, cholesterol, and caveolin-1 were indispensably involved. In addition, lipid raft extraction assay showed that PEDV can also enter cells through lipid raft-mediated endocytosis. To investigate the trafficking of internalized PEDV, we found that PEDV entry into cells relied on low pH and internalized virions reached lysosomes through the early endosome–late endosome–lysosome pathway. The results concretely revealed the entry mechanisms of PEDV and provided an insightful theoretical basis for the further understanding of PEDV pathogenesis and guidance for new targets of antiviral drugs.
has issue date	2020-02-10(xsd:dateTime)
bibo:doi	10.1186/s13567-020-0739-7
bibo:pmid	32041637
has license	cc-by
sha1sum (hex)	35346aa2228281a39b64a6185827e497e57296a3
schema:url	https://doi.org/10.1186/s13567-020-0739-7
resource representing a document's title	PEDV enters cells through clathrin-, caveolae-, and lipid raft-mediated endocytosis and traffics via the endo-/lysosome pathway
has PubMed Central identifier	PMC7011528
has PubMed identifier	32041637
schema:publication	Vet Res
resource representing a document's body	covid:35346aa2228281a39b64a6185827e497e57296a3#body_text
is schema:about of	named entity 'CELLS' named entity 'Given' named entity 'cells' named entity 'virions' named entity 'cells' named entity 'invasion' named entity 'enter' named entity 'endo' named entity 'LIPID RAFT' named entity 'PEDV' named entity 'MEDIATED' named entity 'PATHWAY' named entity 'RESULTS' named entity 'PATHWAY' named entity 'TARGETS' named entity '100%' named entity 'SUBTYPE' named entity 'INVASION' named entity 'HIGHEST' named entity 'GUIDANCE' named entity 'CLATHRIN HEAVY CHAIN' named entity 'FOUND' named entity 'BASIS' named entity 'NEW' named entity 'STUDY' named entity 'GENE' named entity 'DESCRIBED' named entity 'STRAINS' named entity 'FOCUSED' named entity 'PATHOGENIC VARIANT' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'ANTIVIRAL DRUGS' named entity 'MECHANISMS' named entity 'EARLY ENDOSOME' named entity 'LEAD' named entity 'RESEARCH METHODS' named entity 'GIVEN' named entity 'INVOLVED' named entity 'SIGNIFICANT' named entity 'GLOBAL' named entity 'LATE ENDOSOME' named entity 'INDUSTRY' named entity 'LYSOSOMES' named entity 'DYNAMIN II' named entity 'ENTER' named entity 'ASSAY' named entity 'EXTRACTION' named entity 'REVEALED' named entity 'PATHOGENICITY' named entity 'CELLS' named entity 'ENDOCYTOSIS' named entity 'INFORMATION' named entity 'CELLULAR' named entity 'SPECIFIC' named entity 'CHARACTERIZED' named entity 'ADDITION' named entity 'ECONOMIC LOSS' named entity 'THEORETICAL' named entity 'PATHOGENESIS' named entity 'KINETICS' named entity 'USED' named entity 'PATHWAYS' named entity 'HAVE' named entity 'CLARIFY' named entity 'SWINE' named entity 'ENDOCYTOSIS' named entity 'MUTANT STRAINS' named entity 'CLATHRIN' named entity 'ENDO' named entity 'CAVEOLAE' named entity 'BUT' named entity 'LIPID RAFT' named entity 'DIVERSE' named entity 'VERO CELLS' named entity 'VIRIONS' named entity 'CORONAVIRUSES' named entity 'SEQUENCES'

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