About: Effect of co-axially hybridized gene targets on hybridization efficiency of microarrayed DNA probes

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About: Effect of co-axially hybridized gene targets on hybridization efficiency of microarrayed DNA probes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Effect of co-axially hybridized gene targets on hybridization efficiency of microarrayed DNA probes
Creator	Chen, Chia-Chun Huang, Jie-Len Su, Hung-Ju Wu, Jui-Chuang Jiang, Kai
Source	Elsevier; Medline; PMC
abstract	Abstract The effect of relative size of two co-axially hybridized gene targets on the hybridization efficiency was studied for two DNA probe configurations and various probe concentrations. Each of two sets of microarrayed probes contained a pair of DNA probes and a pair of their complementary samples labeled with two distinct fluorescent dyes. The sequence of each probe is especially designed so that two targets are simultaneously complementary to two adjacent sections of the probe. The molecular steric effect on the hybridization efficiency is investigated by comparing the dye signals between configurations of one-target and two-target hybridization scenarios. The results show that a low probe concentration gives better hybridization efficiency and the first-hybridization conducted by a shorter-size DNA target improves the hybridization efficiency of the second target coupling onto the same probe.
has issue date	2011-01-31(xsd:dateTime)
bibo:doi	10.1016/j.jtice.2010.04.012
bibo:pmid	32362954
has license	els-covid
sha1sum (hex)	3441103c5b6729f71a2dea4f93eb7da338c45763
schema:url	https://doi.org/10.1016/j.jtice.2010.04.012
resource representing a document's title	Effect of co-axially hybridized gene targets on hybridization efficiency of microarrayed DNA probes
has PubMed Central identifier	PMC7185593
has PubMed identifier	32362954
schema:publication	Journal of the Taiwan Institute of Chemical Engineers
resource representing a document's body	covid:3441103c5b6729f71a2dea4f93eb7da338c45763#body_text
is schema:about of	named entity 'hybridization' named entity 'EFFICIENCY' named entity 'TARGETS' named entity 'MICROARRAYED' named entity 'EFFECT' named entity 'HYBRIDIZATION' named entity 'GENE' named entity 'DNA PROBES' named entity 'probes' named entity 'hybridized' named entity 'ATP5O' named entity 'CANX' named entity 'hybridization' named entity 'microarray' named entity 'hybridization' named entity 'expression vector' named entity 'cy5' named entity 'DNA probe' named entity 'Cy3' named entity 'XPS' named entity 'hybridized' named entity 'CANX' named entity 'PCR' named entity 'microarrays' named entity 'Taipei' named entity 'single-stranded' named entity 'conformation' named entity 'RNA' named entity 'Japan' named entity 'hybridization' named entity 'hybridization' named entity 'spectroscopy' named entity 'Polymerase Chain Reaction' named entity 'Michigan' named entity 'hybridizers' named entity 'SARS coronavirus' named entity 'experimental design' named entity 'hybridize' named entity 'genomic DNA' named entity 'RNA' named entity 'Taiwan' named entity 'SDS' named entity 'hybridization' named entity 'incubation' named entity 'gene' named entity 'Hsinchu' named entity 'gene' named entity 'phosphorus' named entity 'gene' named entity 'fluorescent' named entity 'Cy5' named entity 'PCR' named entity 'Microarrays' named entity 'CANX' named entity 'glycerol' named entity 'CANX' named entity 'hybridization' named entity 'cDNA' named entity 'Taiwan' named entity 'microtiter plate' named entity 'Invitrogen' named entity 'PSMA5' named entity 'DNA probes' named entity 'experimental design' named entity 'hybridization' named entity 'primers' named entity 'fluorescence' named entity 'fluorescence' named entity 'sodium dodecyl sulfate' named entity 'fluorescent dye' named entity 'glycerol' named entity 'hybridization of probes' named entity 'primer' named entity 'ATP5O' named entity 'DNA probes' named entity 'CANX' named entity 'genome'

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