About: Sugar-Binding Profiles of Chitin-Binding Lectins from the Hevein Family: A Comprehensive Study

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About: Sugar-Binding Profiles of Chitin-Binding Lectins from the Hevein Family: A Comprehensive Study Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Sugar-Binding Profiles of Chitin-Binding Lectins from the Hevein Family: A Comprehensive Study
Creator	Hirabayashi, Jun Itakura, Yoko Kominami, Junko Nakamura-Tsuruta, Sachiko Tateno, Hiroaki
source	PMC
abstract	Chitin-binding lectins form the hevein family in plants, which are defined by the presence of single or multiple structurally conserved GlcNAc (N-acetylglucosamine)-binding domains. Although they have been used as probes for chito-oligosaccharides, their detailed specificities remain to be investigated. In this study, we analyzed six chitin-binding lectins, DSA, LEL, PWM, STL, UDA, and WGA, by quantitative frontal affinity chromatography. Some novel features were evident: WGA showed almost comparable affinity for pyridylaminated chitotriose and chitotetraose, while LEL and UDA showed much weaker affinity, and DSA, PWM, and STL had no substantial affinity for the former. WGA showed selective affinity for hybrid-type N-glycans harboring a bisecting GlcNAc residue. UDA showed extensive binding to high-mannose type N-glycans, with affinity increasing with the number of Man residues. DSA showed the highest affinity for highly branched N-glycans consisting of type II LacNAc (N-acetyllactosamine). Further, multivalent features of these lectins were investigated by using glycoconjugate and lectin microarrays. The lectins showed substantial binding to immobilized LacNAc as well as chito-oligosaccharides, although the extents to which they bound varied among them. WGA showed strong binding to heavily sialylated glycoproteins. The above observations will help interpret lectin-glycoprotein interactions in histochemical studies and glyco-biomarker investigations.
has issue date	2017-05-30(xsd:dateTime)
bibo:doi	10.3390/ijms18061160
bibo:pmid	28556796
has license	cc-by
sha1sum (hex)	3345fa1a77d9ad2f4bce48138490e60a462fe18b
schema:url	https://doi.org/10.3390/ijms18061160
resource representing a document's title	Sugar-Binding Profiles of Chitin-Binding Lectins from the Hevein Family: A Comprehensive Study
has PubMed Central identifier	PMC5485984
has PubMed identifier	28556796
schema:publication	Int J Mol Sci
resource representing a document's body	covid:3345fa1a77d9ad2f4bce48138490e60a462fe18b#body_text
is schema:about of	named entity 'STUDY' named entity 'STUDIES' named entity 'STUDY' named entity 'HAVE' named entity 'FAMILY' named entity 'MAN' named entity 'CONSERVED' named entity 'TYPE' named entity 'GLYCOPROTEINS' named entity 'DSA' named entity 'NOVEL' named entity 'DEFINED' named entity 'LACNAC' named entity 'PWM' named entity 'HYBRID' named entity 'CHITOTRIOSE' named entity 'AFFINITY' named entity 'HEVEIN' named entity 'RESIDUE' named entity 'PLANTS' named entity 'LECTIN' named entity 'SINGLE' named entity 'THEIR' named entity 'OBSERVATIONS' named entity 'MULTIPLE' named entity 'COMPREHENSIVE' named entity 'SPECIFICITIES' named entity 'ALMOST' named entity 'HARBORING' named entity 'CHITIN' named entity 'LECTINS' named entity 'SUGAR' named entity 'HEVEIN' named entity 'MUCH' named entity 'HELP' named entity 'EXTENSIVE' named entity 'PROBES' named entity 'INVESTIGATED' named entity 'DETAILED' named entity 'TYPE II' named entity 'NUMBER OF' named entity 'STL' named entity 'FRONTAL AFFINITY CHROMATOGRAPHY' named entity 'USING' named entity 'GLCNAC' named entity 'SELECTIVE' named entity 'LEL' named entity 'INCREASING' named entity 'FORM' named entity 'EXTENTS' named entity 'STRONG' named entity 'INVESTIGATIONS' named entity 'UDA' named entity 'ACETYLGLUCOSAMINE' named entity 'GLYCOPROTEIN' named entity 'BIOMARKER' named entity 'BRANCHED' named entity 'GLYCOCONJUGATE' named entity 'OLIGOSACCHARIDES' named entity 'INTERACTIONS' named entity 'ANALYZED' named entity 'MICROARRAYS' named entity 'HIGHEST' named entity 'N-GLYCANS' named entity 'CHITOTETRAOSE' named entity 'INTERPRET' named entity 'QUANTITATIVE' named entity 'WGA' named entity 'LECTINS' named entity 'MANNOSE' named entity 'PRESENCE OF' named entity 'CHITIN' named entity 'BINDING' named entity 'BINDING' named entity 'THESE' named entity 'USED' named entity 'FEATURES'

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