About: Expression of SARS-coronavirus envelope protein in Escherichia coli cells alters membrane permeability

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About: Expression of SARS-coronavirus envelope protein in Escherichia coli cells alters membrane permeability Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Expression of SARS-coronavirus envelope protein in Escherichia coli cells alters membrane permeability
Creator	Liao, Y Liu, D Tam, J Lescar, J
Source	Elsevier; Medline; PMC
abstract	Abstract To promote viral entry, replication, release, and spread to neighboring cells, many cytolytic animal viruses encode proteins responsible for modification of host cell membrane permeability and for formation of ion channels in host cell membranes during their life cycles. In this study, we show that the envelope (E) protein of severe acute respiratory syndrome-associated coronavirus can induce membrane permeability changes when expressed in Escherichia coli. E protein expressed in bacterial and mammalian cells under reducing conditions existed as monomers, but formed homodimer and homotrimer under non-reducing conditions. Site-directed mutagenesis studies revealed that two cysteine residues of the E protein were essential for oligomerization, leading to induction of membrane permeability. This is the first report demonstrating that a coronavirus-encoded protein could modify membrane permeability in E. coli cells.
has issue date	2004-12-03(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2004.10.050
bibo:pmid	15522242
has license	els-covid
sha1sum (hex)	3114648f3f6cb30e179902b2a0ad4b25dc64ba74
schema:url	https://doi.org/10.1016/j.bbrc.2004.10.050
resource representing a document's title	Expression of SARS-coronavirus envelope protein in Escherichia coli cells alters membrane permeability
has PubMed Central identifier	PMC7092894
has PubMed identifier	15522242
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:3114648f3f6cb30e179902b2a0ad4b25dc64ba74#body_text
is schema:about of	named entity 'ENVELOPE PROTEIN' named entity 'homodimer' named entity 'residues' named entity 'leading' named entity 'ENVELOPE' named entity 'HOMODIMER' named entity 'FORMED' named entity 'HOST CELL' named entity 'LIFE CYCLES' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'SPREAD' named entity 'MODIFICATION' named entity 'REDUCING' named entity 'CELL MEMBRANES' named entity 'RELEASE' named entity 'CELLS' named entity 'FORMATION' named entity 'ESCHERICHIA COLI' named entity 'NEIGHBORING' named entity 'CELL MEMBRANE PERMEABILITY' named entity 'ASSOCIATED' named entity 'THEIR' named entity 'LEADING' named entity 'BACTERIAL' named entity 'PROTEINS' named entity 'REVEALED' named entity 'OLIGOMERIZATION' named entity 'CORONAVIRUS' named entity 'MEMBRANE PERMEABILITY' named entity 'ENCODE' named entity 'EXPRESSION' named entity 'MEMBRANE PERMEABILITY' named entity 'ESSENTIAL' named entity 'PROMOTE' named entity 'PROTEIN ' named entity 'EXPRESSED' named entity 'CYSTEINE' named entity 'SITE-DIRECTED MUTAGENESIS' named entity 'RESPONSIBLE' named entity 'HOST CELL MEMBRANE' named entity 'INDUCE' named entity 'BUT' named entity 'PROTEIN ' named entity 'SARS' named entity 'CORONAVIRUS' named entity 'CELLS' named entity 'E PROTEIN' named entity 'MONOMERS' named entity 'STUDIES' named entity 'MODIFY' named entity 'CHANGES' named entity 'REPORT' named entity 'MAMMALIAN' named entity 'ENCODED' named entity 'ION CHANNELS' named entity 'REPLICATION' named entity 'ANIMAL VIRUSES' named entity 'VIRAL ENTRY' named entity 'STUDY' named entity 'INDUCTION' named entity 'NON-' named entity 'CONDITIONS' named entity 'E. COLI' named entity 'ESCHERICHIA COLI' named entity 'cell membranes' named entity 'cells' named entity 'membrane permeability' named entity 'protein' named entity 'existed' named entity 'This' named entity 'Site-directed mutagenesis' named entity 'induction' named entity 'demonstrating' named entity 'oligomerization' named entity 'E. coli' named entity 'cells' named entity 'encode proteins' named entity 'cytolytic'

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