About: A Single-Cell Platform for Monitoring Viral Proteolytic Cleavage in Different Cellular Compartments

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About: A Single-Cell Platform for Monitoring Viral Proteolytic Cleavage in Different Cellular Compartments Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Single-Cell Platform for Monitoring Viral Proteolytic Cleavage in Different Cellular Compartments
Creator	Abbadessa, Darin Wolkowicz, Roland Reed, Connor Smurthwaite, Cameron
Source	Medline; PMC
abstract	Infectious diseases affect human health despite advances in biomedical research and drug discovery. Among these, viruses are especially difficult to tackle due to the sudden transfer from animals to humans, high mutational rates, resistance to current treatments, and the intricacies of their molecular interactions with the host. As an example of these interactions, we describe a cell-based approach to monitor specific proteolytic events executed by either the viral-encoded protease or by host proteins on the virus. We then emphasize the significance of examining proteolysis within the subcellular compartment where cleavage occurs naturally. We show the power of stable expression, highlighting the usefulness of the cell-based multiplexed approach, which we have adapted to two independent assays previously developed to monitor (a) the activity of the HIV-1-encoded protease or (b) the cleavage of the HIV-1-encoded envelope protein by the host. Multiplexing was achieved by mixing cells each carrying a different assay or, alternatively, by engineering cells expressing two assays. Multiplexing relies on the robustness of the individual assays and their clear discrimination, further enhancing screening capabilities in an attempt to block proteolytic events required for viral infectivity and spread.
has issue date	2016-09-22(xsd:dateTime)
bibo:doi	10.4137/bci.s30379
bibo:pmid	27688710
has license	no-cc
sha1sum (hex)	30cde71f98e1b4202ba43473e686ec4b5b23d42f
schema:url	https://doi.org/10.4137/bci.s30379
resource representing a document's title	A Single-Cell Platform for Monitoring Viral Proteolytic Cleavage in Different Cellular Compartments
has PubMed Central identifier	PMC5034881
has PubMed identifier	27688710
schema:publication	Biochem Insights
resource representing a document's body	covid:30cde71f98e1b4202ba43473e686ec4b5b23d42f#body_text
is schema:about of	named entity 'sudden' named entity 'assays' named entity 'naturally' named entity 'Living' named entity 'Issue' named entity 'BIOCHEMISTRY' named entity 'MONITORING' named entity 'ENVELOPE PROTEIN' named entity 'THESE' named entity 'CARRYING' named entity 'EITHER' named entity 'PROTEOLYSIS' named entity 'VIRUSES' named entity 'PROTEINS' named entity 'A CELL' named entity 'HIGHLIGHTING' named entity 'HIV-1' named entity 'SCREENING' named entity 'BIOMEDICAL RESEARCH' named entity 'POWER' named entity 'MONITOR' named entity 'ENHANCING' named entity 'MIXING' named entity 'PREVIOUSLY' named entity 'EXAMPLE' named entity 'CURRENT' named entity 'INFECTIVITY' named entity 'HIGH' named entity 'COMPARTMENTS' named entity 'CLEAVAGE' named entity 'CELLULAR' named entity 'INDIVIDUAL' named entity 'MOLECULAR INTERACTIONS' named entity 'DISCRIMINATION' named entity 'DIFFICULT' named entity 'ASSAY' named entity 'INDIVIDUAL' named entity 'SINGLE' named entity 'STABLE EXPRESSION' named entity 'PROTEOLYTIC' named entity 'ADAPTED' named entity 'RATES' named entity 'PLATFORM' named entity 'CELL' named entity 'DIFFERENT' named entity 'ISSUE' named entity 'BASED' named entity 'EXAMINING' named entity 'SUDDEN' named entity 'DESCRIBE' named entity 'VIRUS' named entity 'ACTIVITY' named entity 'MULTIPLEXING' named entity 'COMPARTMENT' named entity 'TREATMENTS' named entity 'CLEAVAGE' named entity 'DUE TO' named entity 'SPREAD' named entity 'BLOCK' named entity 'TRANSFER' named entity 'APPROACH' named entity 'HOST' named entity 'AFFECT' named entity 'WHERE' named entity 'ANIMALS' named entity 'HAVE' named entity 'HUMANS' named entity 'ENCODED' named entity 'USEFULNESS' named entity 'REQUIRED' named entity 'ATTEMPT' named entity '28A' named entity 'EXECUTED' named entity 'INDEPENDENT' named entity 'SIGNIFICANCE' named entity 'EVENTS' named entity 'PROTEIN ' named entity 'INFECTIOUS DISEASES'

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