About: Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles

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About: Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles
Creator	Lee, Kyu-Sung Kim, Jin Kang, Minhee Chung, Doo-Ryeon Hwang, Joonki Kim, Hanbi Park, Minseon
Source	Medline; PMC
abstract	[Image: see text] Worldwide outbreaks of infectious diseases necessitate the development of rapid and accurate diagnostic methods. Colorimetric assays are a representative tool to simply identify the target molecules in specimens through color changes of an indicator (e.g., nanosized metallic particle, and dye molecules). The detection method is used to confirm the presence of biomarkers visually and measure absorbance of the colored compounds at a specific wavelength. In this study, we propose a colorimetric assay based on an extended form of double-stranded DNA (dsDNA) self-assembly shielded gold nanoparticles (AuNPs) under positive electrolyte (e.g., 0.1 M MgCl(2)) for detection of Middle East respiratory syndrome coronavirus (MERS-CoV). This platform is able to verify the existence of viral molecules through a localized surface plasmon resonance (LSPR) shift and color changes of AuNPs in the UV–vis wavelength range. We designed a pair of thiol-modified probes at either the 5′ end or 3′ end to organize complementary base pairs with upstream of the E protein gene (upE) and open reading frames (ORF) 1a on MERS-CoV. The dsDNA of the target and probes forms a disulfide-induced long self-assembled complex, which protects AuNPs from salt-induced aggregation and transition of optical properties. This colorimetric assay could discriminate down to 1 pmol/μL of 30 bp MERS-CoV and further be adapted for convenient on-site detection of other infectious diseases, especially in resource-limited settings.
has issue date	2019-05-07(xsd:dateTime)
bibo:doi	10.1021/acssensors.9b00175
bibo:pmid	31062580
has license	no-cc
sha1sum (hex)	2e13ff4e8fa95d9003faaf8d6358ea4e51a1c5ac
schema:url	https://doi.org/10.1021/acssensors.9b00175
resource representing a document's title	Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles
has PubMed Central identifier	PMC7119221
has PubMed identifier	31062580
schema:publication	ACS Sens
resource representing a document's body	covid:2e13ff4e8fa95d9003faaf8d6358ea4e51a1c5ac#body_text
is schema:about of	named entity 'adapted' named entity 'metallic' named entity 'molecules' named entity 'color' named entity 'compounds' named entity 'MERS-CoV' named entity 'Assay' named entity 'EITHER' named entity 'REPRESENTATIVE' named entity 'SETTINGS' named entity 'RAPID' named entity 'COMPLEX' named entity 'SELF' named entity 'DYE' named entity 'SHIFT' named entity 'ORGANIZE' named entity 'CONFIRM' named entity 'PRESENCE OF' named entity 'PAIR' named entity 'RESOURCE' named entity 'COLORIMETRIC ASSAY' named entity 'MEASURE' named entity 'WAVELENGTH' named entity 'LOCALIZED' named entity 'BASE PAIRS' named entity 'THIOL' named entity 'COLORED' named entity 'DETECTION' covid:arg/2e13ff4e8fa95d9003faaf8d6358ea4e51a1c5ac named entity 'INFECTIOUS DISEASES' named entity 'MERS-COV' named entity 'PARTICLE' named entity 'E PROTEIN' named entity 'PLATFORM' named entity 'EXTENDED' named entity 'OPTICAL PROPERTIES' named entity 'VIRAL' named entity 'MODIFIED' named entity 'INDICATOR' named entity 'AGGREGATION' named entity 'ABSORBANCE' named entity 'IDENTIFY' named entity 'NANOPARTICLES' named entity 'COLORIMETRIC ASSAY' named entity 'USING' named entity 'DEVELOPMENT' named entity 'GOLD' named entity 'LABEL-FREE' named entity 'RANGE' named entity 'END' named entity 'PROTEIN GENE' named entity 'UPSTREAM' named entity 'OTHER INFECTIOUS DISEASES' named entity 'SURFACE PLASMON RESONANCE' named entity 'SITE' named entity 'WORLDWIDE' named entity 'DSDNA' named entity 'POSITIVE' named entity 'DEVELOPMENT' named entity 'ELECTROLYTE' named entity 'DETECTION METHOD' named entity 'FORM' named entity 'CONVENIENT' named entity 'BASED' named entity 'SELF-ASSEMBLY' named entity 'ADAPTED' named entity 'CHANGES' named entity 'INDUCED' named entity 'TARGET' named entity 'DIAGNOSTIC' named entity 'FORMS' named entity 'OPEN READING FRAMES' named entity 'MOLECULES' named entity 'TRANSITION' named entity 'LIMITED'

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