About: SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells

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About: SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells
Creator	Edeen, Karen Wang, Jieru Manzer, Rizwan Jeffers, Scott Wang, Shuanglin Holmes, Kathryn Mason, Robert Miura, Tanya Mossel, Eric Cosgrove, Gregory Funk, C Pearson, Leonard
Source	Elsevier; Medline; PMC
abstract	Abstract Severe acute respiratory syndrome (SARS) is a disease characterized by diffuse alveolar damage. We isolated human alveolar type II cells and maintained them in a highly differentiated state. Type II cell cultures supported SARS-CoV replication as evidenced by RT-PCR detection of viral subgenomic RNA and an increase in virus titer. Virus titers were maximal by 24 h and peaked at approximately 105 pfu/mL. Two cell types within the cultures were infected. One cell type was type II cells, which were positive for SP-A, SP-C, cytokeratin, a type II cell-specific monoclonal antibody, and Ep-CAM. The other cell type was composed of spindle-shaped cells that were positive for vimentin and collagen III and likely fibroblasts. Viral replication was not detected in type I-like cells or macrophages. Hence, differentiated adult human alveolar type II cells were infectible but alveolar type I-like cells and alveolar macrophages did not support productive infection.
has issue date	2008-03-01(xsd:dateTime)
bibo:doi	10.1016/j.virol.2007.09.045
bibo:pmid	18022664
has license	els-covid
sha1sum (hex)	2ca407f8b1a873beeeb6af79620adf02e702e274
schema:url	https://doi.org/10.1016/j.virol.2007.09.045
resource representing a document's title	SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells
has PubMed Central identifier	PMC2312501
has PubMed identifier	18022664
schema:publication	Virology
resource representing a document's body	covid:2ca407f8b1a873beeeb6af79620adf02e702e274#body_text
is schema:about of	named entity 'REPLICATES' named entity 'cell type' named entity 'cell cultures' named entity 'adult' named entity 'cells' named entity 'virus' named entity 'cell types' named entity 'Virus' named entity 'cell cultures' named entity 'human' named entity 'LIKE' named entity 'TYPE II CELL' named entity 'INCREASE' named entity 'CELL TYPES' named entity 'CELL TYPE' named entity 'EP-CAM' named entity 'RNA' named entity 'COMPOSED OF' named entity 'LIKE' named entity 'SPECIFIC' named entity 'NOT DETECTED' named entity 'SPINDLE-SHAPED' named entity 'DID' named entity 'MACROPHAGES' named entity 'DISEASE' named entity 'RT-PCR' named entity 'LIKELY' named entity 'VIRAL' named entity 'SP-C' named entity 'VIMENTIN' named entity 'DIFFERENTIATED' named entity 'COLLAGEN' named entity 'BUT' named entity 'IS A' named entity 'MAXIMAL' named entity 'BUT' named entity 'HUMAN' named entity 'ALVEOLAR TYPE II CELL' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'CHARACTERIZED' named entity 'PEAKED' named entity 'TYPE I' named entity 'SARS-COV' named entity 'PRODUCTIVE' named entity 'CULTURES' named entity 'PFU' named entity 'REPLICATION' named entity 'CELL CULTURES' named entity 'CELLS' named entity 'PRIMARY' named entity 'ONE CELL TYPE' named entity 'FIBROBLASTS' named entity 'MONOCLONAL ANTIBODY' named entity 'CELL CULTURES' named entity 'HUMAN' named entity 'SUPPORT' named entity 'CELLS' named entity 'DETECTION' named entity 'ISOLATED' named entity 'MAINTAINED' named entity 'SUPPORTED' named entity 'VIRUS' named entity 'TITER' named entity 'ADULT' named entity 'STATE' named entity 'TYPE I' named entity 'POSITIVE FOR' named entity 'DIFFUSE ALVEOLAR DAMAGE' named entity 'HIGHLY' named entity 'ALVEOLAR'

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