About: Cytochrome P4501B1 in bone marrow is co-expressed with key markers of mesenchymal stem cells. BMS2 cell line models PAH disruption of bone marrow niche development functions

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Cytochrome P4501B1 in bone marrow is co-expressed with key markers of mesenchymal stem cells. BMS2 cell line models PAH disruption of bone marrow niche development functions Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cytochrome P4501B1 in bone marrow is co-expressed with key markers of mesenchymal stem cells. BMS2 cell line models PAH disruption of bone marrow niche development functions
Creator	Tong, Tiegang Almeldin, Ahmed Jaskula-Sztul, Renata Jefcoate, Colin Larsen, Michele Maguire, Meghan Rondelli, Catherine
Source	Elsevier; Medline; PMC
abstract	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants that are metabolized to carcinogenic dihydrodiol epoxides (PAHDE) by cytochrome P450 1B1 (CYP1B1). This metabolism occurs in bone marrow (BM) mesenchymal stem cells (MSC), which sustain hematopoietic stem and progenitor cells (HSPC). In BM, CYP1B1-mediated metabolism of 7, 12-dimethylbenz[a]anthracene (DMBA) suppresses HSPC colony formation within 6 h, whereas benzo(a)pyrene (BP) generates protective cytokines. MSC, enriched from adherent BM cells, yielded the bone marrow stromal, BMS2, cell line. These cells express elevated basal CYP1B1 that scarcely responds to Ah receptor (AhR) inducers. BMS2 cells exhibit extensive transcriptome overlap with leptin receptor positive mesenchymal stem cells (Lepr+ MSC) that control the hematopoietic niche. The overlap includes CYP1B1 and the expression of HSPC regulatory factors (Ebf3, Cxcl12, Kitl, Csf1 and Gas6). MSC are large, adherent fibroblasts that sequester small HSPC and macrophage in the BM niche (Graphic abstract). High basal CYP1B1 expression in BMS2 cells derives from interactions between the Ah-receptor enhancer and proximal promoter SP1 complexes, boosted by autocrine signaling. PAH effects on BMS2 cells model Lepr+MSC niche activity. CYP1B1 metabolizes DMBA to PAHDE, producing p53-mediated mRNA increases, long after the in vivo HSPC suppression. Faster, direct p53 effects, favored by stem cells, remain possible PAHDE targets. However, HSPC regulatory factors remained unresponsive. BP is less toxic in BMS2 cells, but, in BM, CYP1A1 metabolism stimulates macrophage cytokines (Il1b > Tnfa> Ifng) within 6 h. Although absent from BMS2 and Lepr+MSC, their receptors are highly expressed. The impact of this cytokine signaling in MSC remains to be determined.
has issue date	2020-06-14(xsd:dateTime)
bibo:doi	10.1016/j.taap.2020.115111
bibo:pmid	32553695
has license	no-cc
sha1sum (hex)	2b8faec4658ed847c3e85a89620b24193046017a
schema:url	https://doi.org/10.1016/j.taap.2020.115111
resource representing a document's title	Cytochrome P4501B1 in bone marrow is co-expressed with key markers of mesenchymal stem cells. BMS2 cell line models PAH disruption of bone marrow niche development functions
has PubMed Central identifier	PMC7293885
has PubMed identifier	32553695
schema:publication	Toxicol Appl Pharmacol
resource representing a document's body	covid:2b8faec4658ed847c3e85a89620b24193046017a#body_text
is schema:about of	named entity 'UNRESPONSIVE' named entity 'BUT' named entity 'IN VITRO MODEL' named entity 'APPLIED PHARMACOLOGY' named entity 'PROCESSES' named entity 'cytochrome P450' named entity 'mesenchymal stem cells' named entity 'receptor' named entity 'MSC' named entity 'MSC' named entity 'leptin receptor' named entity 'Cxcl12' named entity 'CYP1B1' named entity 'metabolism' named entity 'CYP1B1' named entity 'p53' named entity 'cytokine receptor' named entity 'metabolism' named entity 'PAH' named entity 'CYP1B1' named entity 'transcriptome' named entity 'macrophage' named entity 'metabolized' named entity 'CYP1B1' named entity 'bone marrow' named entity 'benzo(a)pyrene' named entity 'cytokine' named entity 'Il1b' named entity 'DMBA' named entity 'molecular processes' named entity 'PAH' named entity 'metabolism' named entity 'hematopoietic' named entity 'CYP1B1' named entity 'progenitor cells' named entity 'cell line' named entity 'co-expressed' named entity 'Cytochrome' named entity 'PAH' named entity 'IL1b' named entity 'Cyclin' named entity 'CYP1A1' named entity 'osteoblast' named entity 'AhR' named entity 'IL7' named entity 'DMBA' named entity 'DMBA' named entity 'FZD1' named entity 'basal' named entity 'AhR' named entity 'Mek-Erk' named entity 'lung cancer' named entity 'PAH' named entity 'DMBA' named entity 'Fisher Scientific' named entity 'CYP1A1' named entity 'DMBA' named entity 'PAH' named entity 'p53' named entity 'protein' named entity 'DMBA' named entity 'CYP1B1' named entity 'macrophage' named entity 'chromatin' named entity 'PAH' named entity 'ROS' named entity 'adverse effects' named entity 'TCDD' named entity 'Il1' named entity 'agarose gel electrophoresis' named entity 'Csf1' named entity 'CYP1B1' named entity 'peroxides' named entity 'basal' named entity 'autocrine'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software