About: Recombinant protein‐based ELISA and immuno‐cytochemical assay for the diagnosis of SARS

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About: Recombinant protein‐based ELISA and immuno‐cytochemical assay for the diagnosis of SARS Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Recombinant protein‐based ELISA and immuno‐cytochemical assay for the diagnosis of SARS
Creator	Blasi, Francesco Cassone, Antonio Niedrig, Matthias Carattoli, Alessandra Grasso, Felicia Bonito, Paola Giorgi, Colomba
Source	Medline; PMC
abstract	A new Coronavirus (SARS‐CoV) is the aetiological agent of the severe acute respiratory syndrome (SARS). Because of the critical role played by serological assays for SARS diagnosis, an in‐house ELISA based on SARS‐CoV recombinant antigens was developed. The SARS‐CoV nucleocapsid protein (N), three N fragments (N1, N2, and N3) and the intraviral domain of the membrane protein (M2) were cloned and expressed in Escherichia coli as histidine‐tagged proteins. Six reference sera from SARS patients were used to detect virus‐specific IgG in an ELISA using each recombinant protein as coating antigen. High‐titre positive reactions were detected in all SARS positive sera. The specificity of the assay appears to be high as no positive reaction was detected in the sera of 20 healthy subjects and 73 patients with non‐SARS, low‐tract respiratory infections. Specific hyper‐immune sera to SARS‐CoV and the recombinant proteins, N, N1, N2, N3, and M2 were also generated in mice and rabbits. The specificity of these sera was confirmed by an immunocytochemical assay on biochips of SARS‐CoV infected and uninfected cells. J. Med. Virol. 76:137–142, 2005. © 2005 Wiley‐Liss, Inc.
has issue date	2005-04-15(xsd:dateTime)
bibo:doi	10.1002/jmv.20338
bibo:pmid	15834868
has license	no-cc
sha1sum (hex)	2ad6066a47fc8fed46abea8e3c7058955f4dd5d8
schema:url	https://doi.org/10.1002/jmv.20338
resource representing a document's title	Recombinant protein‐based ELISA and immuno‐cytochemical assay for the diagnosis of SARS
has PubMed Central identifier	PMC7166672
has PubMed identifier	15834868
schema:publication	J Med Virol
resource representing a document's body	covid:2ad6066a47fc8fed46abea8e3c7058955f4dd5d8#body_text
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