About: Single cell BCR and transcriptome analysis after respiratory virus infection reveals spatiotemporal dynamics of antigen-specific B cell responses

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About: Single cell BCR and transcriptome analysis after respiratory virus infection reveals spatiotemporal dynamics of antigen-specific B cell responses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Single cell BCR and transcriptome analysis after respiratory virus infection reveals spatiotemporal dynamics of antigen-specific B cell responses
Creator	Bernasconi, Valentina Yewdell, Jonathan Lycke, Nils Robinson, Jonathan Angeletti, Davide Axelsson, Hannes Borcherding, Nicholas Czarnewski, Paulo Emmanouilidi, Aikaterini Greiff, Victor Harandi, Ali Jayanthan, Jayalal Lebrero-Fernández, Cristina Mathew, Nimitha Nakka, Sravya Smirnov, Ilya Yewdell, William
source	BioRxiv
abstract	B cell responses are a critical component of anti-viral immunity. However, a comprehensive picture of antigen-specific B cell responses, differentiation, clonal proliferation and dynamics in different organs after infection is lacking. Here, we combined single-cell RNA sequencing with single-cell B cell receptor (BCR) characterization of antigen-specific cells in the draining lymph nodes, spleen and lungs after influenza infection. We identify several novel B cell subpopulations forming after infection and find organ-specific differences that persist over the course of the response. We discover important transcriptional differences between memory cells in lungs and lymphoid organs and describe organ-restricted clonal expansion. Strikingly, by combining BCR mutational analysis, monoclonal antibody expression and affinity measurements we find no differences between germinal center (GC)-derived memory and plasmacells, at odds with an affinity-based selection model. By linking antigen-recognition with transcriptional programming, clonal-proliferation and differentiation, these finding provide important advances in our understanding of antiviral B cell immunity.
has issue date	2020-08-24(xsd:dateTime)
bibo:doi	10.1101/2020.08.24.264069
has license	biorxiv
sha1sum (hex)	2745a9a83d406af891f8884a4905d5f8844f0775
schema:url	https://doi.org/10.1101/2020.08.24.264069
resource representing a document's title	Single cell BCR and transcriptome analysis after respiratory virus infection reveals spatiotemporal dynamics of antigen-specific B cell responses
schema:publication	bioRxiv
resource representing a document's body	covid:2745a9a83d406af891f8884a4905d5f8844f0775#body_text
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