About: Expression of 9-O- and 7,9-O-Acetyl Modified Sialic Acid in Cells and Their Effects on Influenza Viruses

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Expression of 9-O- and 7,9-O-Acetyl Modified Sialic Acid in Cells and Their Effects on Influenza Viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Expression of 9-O- and 7,9-O-Acetyl Modified Sialic Acid in Cells and Their Effects on Influenza Viruses
Creator	Weichert, Wendy Parrish, Colin Aguilar, Hector Alford-Lawrence, Brynn Barnard, Karen Buchholz, David Laclair, Justin Wasik, Brian
topic	covid:2448743a5de075f6a1816e46fe72b69cc791da18#this
Source	Medline; PMC
abstract	Sialic acids (Sia) are widely displayed on the surfaces of cells and tissues. Sia come in a variety of chemically modified forms, including those with acetyl modifications at the C-7, C-8, and C-9 positions. Here, we analyzed the distribution and amounts of these acetyl modifications in different human and canine cells. Since Sia or their variant forms are receptors for influenza A, B, C, and D viruses, we examined the effects of these modifications on virus infections. We confirmed that 9-O-acetyl and 7,9-O-acetyl modified Sia are widely but variably expressed across cell lines from both humans and canines. Although they were expressed on the cell surfaces of canine MDCK cell lines, they were located primarily within the Golgi compartment of human HEK-293 and A549 cells. The O-acetyl modified Sia were expressed at low levels of 1 to 2% of total Sia in these cell lines. We knocked out and overexpressed the sialate O-acetyltransferase gene (CasD1) and knocked out the sialate O-acetylesterase gene (SIAE) using CRISPR/Cas9 editing. Knocking out CasD1 removed 7,9-O- and 9-O-acetyl Sia expression, confirming previous reports. However, overexpression of CasD1 and knockout of SIAE gave only modest increases in 9-O-acetyl levels in cells and no change in 7,9-O-acetyl levels, indicating that there are complex regulations of these modifications. These modifications were essential for influenza C and D infection but had no obvious effect on influenza A and B infection.
has issue date	2019-12-03(xsd:dateTime)
bibo:doi	10.1128/mbio.02490-19
bibo:pmid	31796537
has license	cc-by
sha1sum (hex)	2448743a5de075f6a1816e46fe72b69cc791da18
schema:url	https://doi.org/10.1128/mbio.02490-19
resource representing a document's title	Expression of 9-O- and 7,9-O-Acetyl Modified Sialic Acid in Cells and Their Effects on Influenza Viruses
has PubMed Central identifier	PMC6890989
has PubMed identifier	31796537
schema:publication	mBio
resource representing a document's body	covid:2448743a5de075f6a1816e46fe72b69cc791da18#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/2448743a5de075f6a1816e46fe72b69cc791da18
is schema:about of	named entity 'EXPRESSION' named entity 'Sia' named entity 'infections' named entity 'human' named entity 'knocked' named entity 'SIAE' named entity 'Sia' named entity 'overexpression' named entity 'influenza' named entity 'SIALATE O-ACETYLESTERASE' named entity 'EXPRESSED' named entity 'HEK-293' named entity 'EFFECTS' named entity 'BEING' named entity 'CASD1' named entity 'SIALIC ACID' named entity 'INFLUENZA VIRUSES' named entity 'DISPLAYED' named entity 'GAVE' named entity 'REGULATIONS' named entity 'GOLGI' named entity 'VARIANT' named entity 'CELLS' named entity 'THEIR' named entity 'EFFECT' named entity 'INFLUENZA' named entity 'INFLUENZA VIRUS' named entity 'REMOVED' named entity 'ESSENTIAL' named entity 'CHANGE IN' named entity 'TOTAL' named entity 'COME' named entity 'THESE' named entity 'PRODUCE' named entity 'VARIETY' named entity 'KNOCKING OUT' named entity 'FORMS' named entity 'INVOLVED' named entity 'TISSUES' named entity 'CONFIRMING' named entity 'SIAE' named entity 'ENZYMES' named entity 'NO CHANGE' named entity 'MODEST' named entity 'EFFECTS' named entity 'ACETYL' named entity 'MODIFIED' named entity 'INFLUENZA A' named entity 'ENGINEERING' named entity 'THEIR' named entity 'SIALIC ACIDS' named entity 'VIRUSES' named entity 'MODIFIED' named entity 'HUMAN' named entity 'HERE' named entity 'KEY' named entity 'EDITING' named entity 'KNOCKED OUT' named entity 'LEVELS' named entity 'CRISPR' named entity 'EXPRESSION' named entity 'GROUPS' named entity 'GLYCANS' named entity 'MDCK CELL' named entity 'ANALYZED' named entity 'DIFFERENT' named entity 'RESEARCH' named entity 'OVEREXPRESSION' named entity 'USING' named entity 'CELL LINES' named entity 'ACROSS' named entity 'INCREASES'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software