About: Saikosaponin C exerts anti-HBV effects by attenuating HNF1α and HNF4α expression to suppress HBV pgRNA synthesis

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Saikosaponin C exerts anti-HBV effects by attenuating HNF1α and HNF4α expression to suppress HBV pgRNA synthesis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Saikosaponin C exerts anti-HBV effects by attenuating HNF1α and HNF4α expression to suppress HBV pgRNA synthesis
Creator	Yuan, Jing Sun, Lina Ding, Xiaoyan Ke, Zhiyi Pan, Yanchao Shen, Yun Ye, Hong Zhang, Runze
topic	covid:2335bfb84be2b9c405b758a4f8f96627d20ece65#this
Source	Medline; PMC
abstract	OBJECTIVE: Saikosaponin c (SSc), a compound purified from the traditional Chinese herb of Radix Bupleuri was previously identified to exhibit anti-HBV replication activity. However, the mechanism through which SSc acts against HBV remains unknown. In this study, we investigated the mechanism of SSc mediated anti-HBV activity. METHODS: HepG2.2.15 cells were cultured at 37 ℃ in the presence of 1–40 μg/mL of SSc or DMSO as a control. The expression profile of HBV markers, cytokines, HNF1α and HNF4α were investigated by real-time quantitative PCR, Elisa, Western blot and Dot blotting. Knockdown of HNF1α or HNF4α in HepG2.2.15 cells was mediated by two small siRNAs specifically targeting HNF1α or HNF4α. RESULTS: We found that SSc stimulates IL-6 expression, leading to attenuated HNF1α and HNF4α expression, which further mediates suppression of HBV pgRNA synthesis. Knockdown of HNF1α or HNF4α in HepG2.2.15 cells by RNA interference abrogates SSc’s anti-HBV role. Moreover, SSc is effective to both wild-type and drug-resistant HBV mutants. CONCLUSION: SSc inhibits pgRNA synthesis by targeting HNF1α and HNF4α. These results indicate that SSc acts as a promising compound for modulating pgRNA transcription in the therapeutic strategies against HBV infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00011-019-01284-2) contains supplementary material, which is available to authorized users.
has issue date	2019-09-17(xsd:dateTime)
bibo:doi	10.1007/s00011-019-01284-2
bibo:pmid	31531682
has license	no-cc
sha1sum (hex)	2335bfb84be2b9c405b758a4f8f96627d20ece65
schema:url	https://doi.org/10.1007/s00011-019-01284-2
resource representing a document's title	Saikosaponin C exerts anti-HBV effects by attenuating HNF1α and HNF4α expression to suppress HBV pgRNA synthesis
has PubMed Central identifier	PMC7079752
has PubMed identifier	31531682
schema:publication	Inflamm Res
resource representing a document's body	covid:2335bfb84be2b9c405b758a4f8f96627d20ece65#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/2335bfb84be2b9c405b758a4f8f96627d20ece65
is schema:about of	named entity 'EXERTS' named entity 'SSc' named entity 'cytokines' named entity 'blotting' named entity 'cells' named entity 'inhibits' named entity 'attenuated' named entity 'HNF1α' named entity 'HNF1α' named entity 'HBV' named entity 'EXPRESSION' named entity 'HNF1' named entity 'MECHANISM' named entity 'ACTS' named entity 'DRUG' named entity 'RADIX' named entity 'CHINESE HERB' named entity 'SMALL' named entity 'CONTROL' named entity 'ELISA' named entity 'PRESENCE OF' named entity 'STUDY' named entity 'THESE' named entity 'PGRNA' named entity 'CULTURED' named entity 'WILD-TYPE' named entity 'SUPPRESSION' named entity 'EFFECTIVE' named entity 'RNA INTERFERENCE' named entity 'METHODS' named entity '27S' named entity 'A COMPOUND' named entity 'INVESTIGATED' named entity 'PURIFIED' named entity 'HNF4' named entity 'UNKNOWN' named entity 'WESTERN BLOT' named entity 'ROLE' named entity 'RESULTS' named entity 'HNF1' named entity 'CONCLUSION' named entity 'MODULATING' named entity 'REPLICATION' named entity 'BCG' named entity 'FOUND' named entity 'TARGETING' named entity 'SAIKOSAPONIN C' named entity 'PREVIOUSLY' named entity 'COMPOUND' named entity 'MARKERS' named entity 'HBV INFECTION' named entity 'IL-6' named entity 'SAIKOSAPONIN C' named entity 'SUPPRESS' named entity 'EFFECTS' named entity 'HNF4' named entity 'SYNTHESIS' named entity 'RESISTANT' named entity 'EXHIBIT' named entity 'THERAPEUTIC' named entity 'MEDIATED' named entity 'DMSO' named entity 'STRATEGIES' named entity 'MUTANTS' named entity 'LEADING' named entity 'SYNTHESIS' named entity 'ATTENUATED' named entity 'TRANSCRIPTION' named entity 'DOT BLOTTING' named entity 'CYTOKINES' named entity 'TRADITIONAL' named entity 'ACTIVITY'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software