About: Development of real-time recombinase polymerase amplification assay for rapid and sensitive detection of canine parvovirus 2

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About: Development of real-time recombinase polymerase amplification assay for rapid and sensitive detection of canine parvovirus 2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of real-time recombinase polymerase amplification assay for rapid and sensitive detection of canine parvovirus 2
Creator	Lu, Yan Liu, Libing Wang, Jianchang Chang, Yan-Zhong Geng, Yunyun Tan, Ke
Source	Medline; PMC
abstract	BACKGROUND: Canine parvovirus 2, a linear single-stranded DNA virus belonging to the genus Parvovirus within the family Parvoviridae, is a highly contagious pathogen of domestic dogs and several wild canidae species. Early detection of canine parvovirus (CPV-2) is crucial to initiating appropriate outbreak control strategies. Recombinase polymerase amplification (RPA), a novel isothermal gene amplification technique, has been developed for the molecular detection of diverse pathogens. In this study, a real-time RPA assay was developed for the detection of CPV-2 using primers and an exo probe targeting the CPV-2 nucleocapsid protein gene. RESULTS: The real-time RPA assay was performed successfully at 38 °C, and the results were obtained within 4–12 min for 10(5)–10(1) molecules of template DNA. The assay only detected CPV-2, and did not show cross-detection of other viral pathogens, demonstrating a high level of specificity. The analytical sensitivity of the real-time RPA was 10(1) copies/reaction of a standard DNA template, which was 10 times more sensitive than the common RPA method. The clinical sensitivity of the real-time RPA assay matched 100% (n = 91) to the real-time PCR results. CONCLUSION: The real-time RPA assay is a simple, rapid, reliable and affordable method that can potentially be applied for the detection of CPV-2 in the research laboratory and point-of-care diagnosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-017-1232-z) contains supplementary material, which is available to authorized users.
has issue date	2017-11-06(xsd:dateTime)
bibo:doi	10.1186/s12917-017-1232-z
bibo:pmid	29110666
has license	cc-by
sha1sum (hex)	225d47cea0dca6f6211b16f9bf8911e2360fb75b
schema:url	https://doi.org/10.1186/s12917-017-1232-z
resource representing a document's title	Development of real-time recombinase polymerase amplification assay for rapid and sensitive detection of canine parvovirus 2
has PubMed Central identifier	PMC5674863
has PubMed identifier	29110666
schema:publication	BMC Vet Res
resource representing a document's body	covid:225d47cea0dca6f6211b16f9bf8911e2360fb75b#body_text
is schema:about of	named entity 'DEVELOPMENT' named entity 'reliable' named entity 'molecular' named entity 'DNA' named entity 'amplification' named entity 'belonging' named entity 'CPV' named entity 'pathogen' named entity 'CPV' named entity 'sensitivity' named entity 'real-time PCR' named entity 'REAL-TIME' named entity 'DID' named entity 'COMMON' named entity '100%' named entity 'RECOMBINASE' named entity 'RESEARCH LABORATORY' named entity 'RAPID' named entity 'POLYMERASE' named entity 'PRIMERS' named entity 'AMPLIFICATION' named entity 'LINEAR' named entity 'REACTION' named entity 'REAL-TIME PCR ' named entity 'ASSAY' named entity 'RELIABLE' named entity 'OUTBREAK CONTROL' named entity 'MOLECULES' named entity 'APPROPRIATE' named entity 'HIGHLY' named entity 'CANINE PARVOVIRUS' named entity 'RPA' named entity 'STANDARD' named entity 'SENSITIVE' named entity 'EXO' named entity 'POLYMERASE' named entity 'SENSITIVE' named entity 'ASSAY' named entity 'DETECTION' named entity 'BELONGING' named entity 'POINT-OF-CARE' named entity 'CANINE PARVOVIRUS' named entity 'DETECTION' named entity 'VIRAL PATHOGENS' named entity 'COPIES' named entity 'NOVEL' named entity 'PERFORMED' named entity 'GENUS PARVOVIRUS' named entity 'BACKGROUND' named entity 'APPLIED' named entity 'RECOMBINASE' named entity 'USING' named entity 'DETECTED' named entity 'SIMPLE' named entity 'RESULTS' named entity 'INITIATING' named entity 'GENE AMPLIFICATION TECHNIQUE' named entity 'PATHOGENS' named entity 'CROSS' named entity 'ANALYTICAL' named entity 'REAL-TIME' named entity 'EARLY DETECTION' named entity 'CLINICAL SENSITIVITY' named entity 'SPECIES' named entity 'PROBE' named entity 'DIVERSE' named entity 'TIMES' named entity 'STUDY' named entity 'PATHOGEN' named entity 'DOMESTIC' named entity 'CPV' named entity 'DOGS' named entity 'TARGETING' named entity 'CANIDAE' named entity 'HIGH LEVEL' named entity 'SENSITIVITY'

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