About: SARS coronavirus papain-like protease induces Egr-1-dependent up-regulation of TGF-β1 via ROS/p38 MAPK/STAT3 pathway

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About: SARS coronavirus papain-like protease induces Egr-1-dependent up-regulation of TGF-β1 via ROS/p38 MAPK/STAT3 pathway Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS coronavirus papain-like protease induces Egr-1-dependent up-regulation of TGF-β1 via ROS/p38 MAPK/STAT3 pathway
Creator	Li, Shih-Wein Lin, Cheng-Wen Lin, Ying-Ju Wan, Lei Huang, Su-Hua Jou, Yu-Jen Wang, Ching-Ying Yang, Tsuey-Ching
Source	Medline; PMC
abstract	SARS coronavirus (SARS-CoV) papain-like protease (PLpro) has been identified in TGF-β1 up-regulation in human promonocytes (Proteomics 2012, 12: 3193-205). This study investigates the mechanisms of SARS-CoV PLpro-induced TGF-β1 promoter activation in human lung epithelial cells and mouse models. SARS-CoV PLpro dose- and time-dependently up-regulates TGF-β1 and vimentin in A549 cells. Dual luciferase reporter assays with TGF-β1 promoter plasmids indicated that TGF-β1 promoter region between −175 to −60, the Egr-1 binding site, was responsible for TGF-β1 promoter activation induced by SARS-CoV PLpro. Subcellular localization analysis of transcription factors showed PLpro triggering nuclear translocation of Egr-1, but not NF-κB and Sp-1. Meanwhile, Egr-1 silencing by siRNA significantly reduced PLpro-induced up-regulation of TGF-β1, TSP-1 and pro-fibrotic genes. Furthermore, the inhibitors for ROS (YCG063), p38 MAPK (SB203580), and STAT3 (Stattic) revealed ROS/p38 MAPK/STAT3 pathway involving in Egr-1 dependent activation of TGF-β1 promoter induced by PLpro. In a mouse model with a direct pulmonary injection, PLpro stimulated macrophage infiltration into lung, up-regulating Egr-1, TSP-1, TGF-β1 and vimentin expression in lung tissues. The results revealed that SARS-CoV PLpro significantly triggered Egr-1 dependent activation of TGF-β1 promoter via ROS/p38 MAPK/STAT3 pathway, correlating with up-regulation of pro-fibrotic responses in vitro and in vivo.
has issue date	2016-05-13(xsd:dateTime)
bibo:doi	10.1038/srep25754
bibo:pmid	27173006
has license	cc-by
sha1sum (hex)	222534164391422210b3089109605e8ff4d1d46c
schema:url	https://doi.org/10.1038/srep25754
resource representing a document's title	SARS coronavirus papain-like protease induces Egr-1-dependent up-regulation of TGF-β1 via ROS/p38 MAPK/STAT3 pathway
has PubMed Central identifier	PMC4865725
has PubMed identifier	27173006
schema:publication	Sci Rep
resource representing a document's body	covid:222534164391422210b3089109605e8ff4d1d46c#body_text
is schema:about of	named entity 'LIKE' named entity 'revealed' named entity 'induced' named entity 'macrophage' named entity 'TGF-β1' named entity 'revealed' named entity 'dependent' named entity 'Egr-1' named entity 'SARS-CoV' named entity 'ROS' named entity 'DEPENDENT' covid:arg/222534164391422210b3089109605e8ff4d1d46c named entity 'SARS-COV' named entity 'ROS' named entity 'INDUCED' named entity 'TRIGGERING' named entity 'BINDING SITE' named entity 'RESULTS' named entity 'BUT' named entity 'PULMONARY' named entity 'REGULATING' named entity 'BAB' named entity 'STAT3' named entity 'SIRNA' named entity 'SUBCELLULAR LOCALIZATION' named entity 'FIBROTIC' named entity 'ACTIVATION' named entity 'INDICATED' named entity 'DEPENDENT' named entity 'SILENCING' named entity 'IN VITRO' named entity 'INVOLVING' named entity 'B21' named entity 'ANALYSIS' named entity 'RESPONSES' named entity 'PLASMIDS' named entity 'INHIBITORS' named entity 'NUCLEAR TRANSLOCATION' named entity 'EGR-1' named entity 'GENES' named entity 'REDUCED' named entity 'PRO-' named entity 'CELLS' named entity 'TSP-1' named entity 'A MOUSE' named entity 'LUNG' named entity 'PROTEASE' named entity 'P38 MAPK' named entity 'OPEN' named entity 'SARS CORONAVIRUS' named entity 'TGF' named entity 'PAPAIN' named entity 'PATHWAY' named entity 'B21' named entity 'ROS' named entity 'TGF' named entity 'DUAL' named entity 'DIRECT' named entity 'EXPRESSION' named entity 'REPORTER' named entity 'VIMENTIN' named entity 'PROMOTER' named entity '9260' named entity 'MAPK' named entity 'TRANSCRIPTION FACTORS' named entity 'MACROPHAGE INFILTRATION' named entity 'INJECTION' named entity 'P38 MAPK' named entity 'UP-REGULATION' named entity 'EGR-1' named entity 'RESPONSIBLE' named entity 'MOUSE MODEL' named entity 'LUCIFERASE' named entity 'LUNG TISSUES'

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