About: SARS coronavirus nucleocapsid immunodominant T-cell epitope cluster is common to both exogenous recombinant and endogenous DNA-encoded immunogens

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About: SARS coronavirus nucleocapsid immunodominant T-cell epitope cluster is common to both exogenous recombinant and endogenous DNA-encoded immunogens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS coronavirus nucleocapsid immunodominant T-cell epitope cluster is common to both exogenous recombinant and endogenous DNA-encoded immunogens
Creator	Brusic, Vladimir Yang, Kun Chikhlikar, Priya Sun, Kai Anwar, Azlinda Chandrasekaran, Ananth Gupta, Vandana Salmon, Jerome Marques, Ernesto August, Thomas Kellathur, Srinivasan Tabiin, Tani
Source	Elsevier; Medline; PMC
abstract	Abstract Correspondence between the T-cell epitope responses of vaccine immunogens and those of pathogen antigens is critical to vaccine efficacy. In the present study, we analyzed the spectrum of immune responses of mice to three different forms of the SARS coronavirus nucleocapsid (N): (1) exogenous recombinant protein (N-GST) with Freund's adjuvant; (2) DNA encoding unmodified N as an endogenous cytoplasmic protein (pN); and (3) DNA encoding N as a LAMP-1 chimera targeted to the lysosomal MHC II compartment (p-LAMP-N). Lysosomal trafficking of the LAMP/N chimera in transfected cells was documented by both confocal and immunoelectron microscopy. The responses of the immunized mice differed markedly. The strongest T-cell IFN-γ and CTL responses were to the LAMP-N chimera followed by the pN immunogen. In contrast, N-GST elicited strong T cell IL-4 but minimal IFN-γ responses and a much greater antibody response. Despite these differences, however, the immunodominant T-cell ELISpot responses to each of the three immunogens were elicited by the same N peptides, with the greatest responses being generated by a cluster of five overlapping peptides, N76–114, each of which contained nonameric H2d binding domains with high binding scores for both class I and, except for N76–93, class II alleles. These results demonstrate that processing and presentation of N, whether exogenously or endogenously derived, resulted in common immunodominant epitopes, supporting the usefulness of modified antigen delivery and trafficking forms and, in particular, LAMP chimeras as vaccine candidates. Nevertheless, the profiles of T-cell responses were distinctly different. The pronounced Th-2 and humoral response to N protein plus adjuvant are in contrast to the balanced IFN-γ and IL-4 responses and strong memory CTL responses to the LAMP-N chimera.
has issue date	2006-03-30(xsd:dateTime)
bibo:doi	10.1016/j.virol.2005.11.042
bibo:pmid	16387339
has license	els-covid
sha1sum (hex)	2141f65d307159d96e111b2f68dff9fe7499054e
schema:url	https://doi.org/10.1016/j.virol.2005.11.042
resource representing a document's title	SARS coronavirus nucleocapsid immunodominant T-cell epitope cluster is common to both exogenous recombinant and endogenous DNA-encoded immunogens
has PubMed Central identifier	PMC7111852
has PubMed identifier	16387339
schema:publication	Virology
resource representing a document's body	covid:2141f65d307159d96e111b2f68dff9fe7499054e#body_text
is schema:about of	named entity 'mice' named entity 'peptides' named entity 'contrast' named entity 'LAMP' named entity 'IFN-g' named entity 'LAMP' named entity 'protein' named entity '28P' named entity 'CTL' named entity 'PRESENTATION' named entity 'DOCUMENTED' named entity 'BALANCED' named entity 'ANTIGENS' named entity 'THESE' named entity 'ENDOGENOUS' named entity 'LAMP-1' named entity 'ANTIBODY RESPONSE' named entity 'RECOMBINANT PROTEIN' named entity 'EXOGENOUS' named entity 'OVERLAPPING' named entity 'CELLS' named entity 'GST' named entity 'STUDY' named entity 'CYTOPLASMIC PROTEIN' named entity 'EPITOPE' named entity 'CORRESPONDENCE' named entity 'CRITICAL' named entity 'RESULTS' named entity 'PROTEIN ' named entity 'BINDING' named entity 'FOLLOWED BY' named entity 'IMMUNOELECTRON MICROSCOPY' named entity 'GENERATED' named entity 'DIFFERENT' named entity 'FORMS' named entity 'MHC' named entity 'SPECTRUM' named entity 'PRESENT' named entity 'IMMUNIZED' named entity 'PATHOGEN' named entity 'BUT' named entity '114' named entity 'VACCINE' named entity 'PROCESSING' named entity 'ADJUVANT' named entity 'DNA' covid:arg/2141f65d307159d96e111b2f68dff9fe7499054e named entity 'ELICITED' named entity 'GREATER' named entity 'BEING' named entity 'ANTIGEN DELIVERY' named entity '283' named entity 'NUCLEOCAPSID' named entity 'MICE' named entity 'DERIVED' named entity 'ANALYZED' named entity 'EXCEPT FOR' named entity 'STRONG' named entity 'IMMUNE RESPONSES' named entity 'LAMP' named entity 'IMMUNODOMINANT EPITOPES' named entity 'MUCH' named entity 'VACCINE EFFICACY' named entity '27S' named entity 'TARGETED' named entity 'T CELL' named entity 'COMMON' named entity 'ENCODING' named entity 'IMMUNOGENS' named entity 'CONTAINED'

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