About: Reference Genes for the Normalization of Gene Expression in Eucalyptus Species

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Reference Genes for the Normalization of Gene Expression in Eucalyptus Species
Creator	Abruzzi De Oliveira, Luisa Breton, Michèle Camargo, Silva Da, Sandro Frazzon, Jeverson Macedo Bastolla, Fernanda Margis, Rogério Pasquali, Giancarlo
Source	Medline; PMC
abstract	Gene expression analysis is increasingly important in biological research, with reverse transcription–quantitative PCR (RT–qPCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Considering the increased sensitivity, reproducibility and large dynamic range of this method, the requirements for proper internal reference gene(s) for relative expression normalization have become much more stringent. Given the increasing interest in the functional genomics of Eucalyptus, we sought to identify and experimentally verify suitable reference genes for the normalization of gene expression associated with the flower, leaf and xylem of six species of the genus. We selected 50 genes that exhibited the least variation in microarrays of E. grandis leaves and xylem, and E. globulus xylem. We further performed the experimental analysis using RT–qPCR for six Eucalyptus species and three different organs/tissues. Employing algorithms geNorm and NormFinder, we assessed the gene expression stability of eight candidate new reference genes. Classic housekeeping genes were also included in the analysis. The stability profiles of candidate genes were in very good agreement. PCR results proved that the expression of novel Eucons04, Eucons08 and Eucons21 genes was the most stable in all Eucalyptus organs/tissues and species studied. We showed that the combination of these genes as references when measuring the expression of a test gene results in more reliable patterns of expression than traditional housekeeping genes. Hence, novel Eucons04, Eucons08 and Eucons21 genes are the best suitable references for the normalization of expression studies in the Eucalyptus genus.
has issue date	2011-12-24(xsd:dateTime)
bibo:doi	10.1093/pcp/pcr187
bibo:pmid	22197885
has license	no-cc
sha1sum (hex)	20552486da880c61b120a7a966f1ba4bcbeb8702
schema:url	https://doi.org/10.1093/pcp/pcr187
resource representing a document's title	Reference Genes for the Normalization of Gene Expression in Eucalyptus Species
has PubMed Central identifier	PMC7107212
has PubMed identifier	22197885
schema:publication	Plant Cell Physiol
resource representing a document's body	covid:20552486da880c61b120a7a966f1ba4bcbeb8702#body_text
is schema:about of	named entity 'GENE EXPRESSION' named entity 'Eucalyptus' named entity 'expression' named entity 'leaves' named entity 'normalization' named entity 'VERY GOOD' named entity 'DIFFERENT' named entity 'THROUGHPUT' named entity 'ACCURATE' named entity 'EXPRESSION PROFILING' named entity 'PROPER' named entity 'EUCALYPTUS' named entity 'ASSOCIATED WITH' named entity 'STABILITY' named entity 'GENES' named entity 'GENUS' named entity 'RT-QPCR' named entity 'HIGH' named entity 'CLASSIC' covid:arg/20552486da880c61b120a7a966f1ba4bcbeb8702 named entity 'IMPORTANT' named entity 'DYNAMIC RANGE' named entity 'LEAVES' named entity 'VERIFY' named entity 'MICROARRAYS' named entity 'BIOLOGICAL RESEARCH' named entity 'TEST' named entity 'VARIATION' named entity 'INCREASING' named entity 'PCR' named entity 'SUITABLE' named entity 'ASSESSED' named entity 'REQUIREMENTS' named entity 'NEW' named entity 'MEASURING' named entity 'METHOD' named entity 'HOUSEKEEPING GENES' named entity 'THESE' named entity 'BEST' named entity 'REPRODUCIBILITY' named entity 'EUCALYPTUS' named entity 'REFERENCE' named entity 'FLOWER' named entity 'GENE EXPRESSION ANALYSIS' named entity 'QUANTITATIVE PCR' named entity '28S' named entity 'PATTERNS' named entity 'PERFORMED' named entity 'REFERENCE GENE' named entity 'HAVE' named entity 'MUCH' named entity 'XYLEM' named entity 'EXPRESSION' named entity 'GENE' named entity 'ALGORITHMS' named entity 'FUNCTIONAL GENOMICS' named entity 'QPCR' named entity 'INCLUDED' named entity 'CONSIDERING' named entity 'CANDIDATE' named entity 'GENE EXPRESSION' named entity 'SPECIES' named entity 'STABLE' named entity 'SPECIES' named entity 'GENES' named entity 'NORMALIZATION' named entity 'RELIABLE' named entity 'AGREEMENT' named entity 'LEAF' named entity 'RESULTS' named entity 'REFERENCES' named entity 'USING' named entity 'REFERENCE' named entity 'TRADITIONAL'

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