About: Molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (Oncorhynchus mykiss)

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About: Molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (Oncorhynchus mykiss) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (Oncorhynchus mykiss)
Creator	Delwart, Eric Katona, Ryan Leis, Eric Fei, Terry Ng, Fan Waltzek, Thomas Batts, William Breyta, Rachel Brieuc, Marine Conway, Carla Lapatra, Scott Purcell, Maureen Winton, James
source	Elsevier; Medline; PMC
abstract	Abstract A novel virus, rainbow trout orthomyxovirus (RbtOV), was isolated in 1997 and again in 2000 from commercially-reared rainbow trout (Oncorhynchus mykiss) in Idaho, USA. The virus grew optimally in the CHSE-214 cell line at 15°C producing a diffuse cytopathic effect; however, juvenile rainbow trout exposed to cell culture-grown virus showed no mortality or gross pathology. Electron microscopy of preparations from infected cell cultures revealed the presence of typical orthomyxovirus particles. The complete genome of RbtOV is comprised of eight linear segments of single-stranded, negative-sense RNA having highly conserved 5′ and 3′-terminal nucleotide sequences. Another virus isolated in 2014 from steelhead trout (also O. mykiss) in Wisconsin, USA, and designated SttOV was found to have eight genome segments with high amino acid sequence identities (89–99%) to the corresponding genes of RbtOV, suggesting these new viruses are isolates of the same virus species and may be more widespread than currently realized. The new isolates had the same genome segment order and the closest pairwise amino acid sequence identities of 16–42% with Infectious salmon anemia virus (ISAV), the type species and currently only member of the genus Isavirus in the family Orthomyxoviridae. However, pairwise comparisons of the predicted amino acid sequences of the 10 RbtOV and SttOV proteins with orthologs from representatives of the established orthomyxoviral genera and a phylogenetic analysis using the PB1 protein showed that while RbtOV and SttOV clustered most closely with ISAV, they diverged sufficiently to merit consideration as representatives of a novel genus. A set of PCR primers was designed using conserved regions of the PB1 gene to produce amplicons that may be sequenced for identification of similar fish orthomyxoviruses in the future.
has issue date	2017-02-15(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2017.01.005
bibo:pmid	28088362
has license	els-covid
sha1sum (hex)	1b3f9daeb1b6fa660774ccef8f9e545ceaf7bdc9
schema:url	https://doi.org/10.1016/j.virusres.2017.01.005
resource representing a document's title	Molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (Oncorhynchus mykiss)
has PubMed Central identifier	PMC7111338
has PubMed identifier	28088362
schema:publication	Virus Research
resource representing a document's body	covid:1b3f9daeb1b6fa660774ccef8f9e545ceaf7bdc9#body_text
is schema:about of	named entity 'virus' named entity 'isolated' named entity 'clustered' named entity 'preparations' named entity 'rainbow trout' named entity 'segments' named entity '80%' named entity 'WIDESPREAD' named entity 'ELECTRON MICROSCOPY' named entity 'CLUSTERED' named entity 'THESE' named entity 'IDENTIFICATION' named entity 'TROUT' named entity 'INFECTIOUS SALMON ANEMIA VIRUS' named entity 'SEGMENTS' named entity 'CELL LINE' named entity 'IDAHO' named entity '1997' named entity 'SPECIES' named entity 'PCR PRIMERS' named entity 'VIRUSES' named entity 'CELL CULTURES' named entity 'GROSS PATHOLOGY' named entity 'ISOLATES' covid:arg/1b3f9daeb1b6fa660774ccef8f9e545ceaf7bdc9 named entity 'MOLECULAR' named entity 'CHARACTERIZATION' named entity 'CURRENTLY' named entity 'DIFFUSE' named entity 'HIGH' named entity '2889' named entity 'LINEAR' named entity 'RAINBOW' named entity 'TROUT' named entity 'ORTHOMYXOVIRUS' named entity 'FAMILY ORTHOMYXOVIRIDAE' named entity 'FISH' named entity 'TO PRODUCE' named entity 'SIMILAR' named entity 'NEGATIVE' named entity 'NUCLEOTIDE SEQUENCES' named entity 'ESTABLISHED' named entity 'MERIT' named entity 'CLOSEST' named entity 'COMPLETE' named entity 'JUVENILE' named entity 'PARTICLES' named entity 'MORTALITY' named entity 'WISCONSIN' named entity 'TYPICAL' named entity 'PROTEIN ' named entity 'NOVEL' named entity 'STEELHEAD' named entity '2000' named entity 'ORTHOMYXOVIRUS' named entity 'TERMINAL' named entity 'GENE' named entity 'RNA' named entity 'REVEALED' named entity 'MEMBER OF' named entity 'VIRUS' named entity 'GENUS' named entity 'PREDICTED' named entity 'IDENTITIES' named entity 'TYPE' named entity 'ORTHOMYXOVIRUSES' named entity 'PRODUCING' named entity 'PRESENCE OF' named entity 'ISOLATED'

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