About: Proteomic comparison of prostate cancer cell lines LNCaP‐FGC and LNCaP‐r reveals heatshock protein 60 as a marker for prostate malignancy

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About: Proteomic comparison of prostate cancer cell lines LNCaP‐FGC and LNCaP‐r reveals heatshock protein 60 as a marker for prostate malignancy Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Proteomic comparison of prostate cancer cell lines LNCaP‐FGC and LNCaP‐r reveals heatshock protein 60 as a marker for prostate malignancy
Creator	Bergh, Anders Bergman, Tomas Byman, Irene Chuan, Yin-Choy Ke Pousette, Å Norstedt, Gunnar Pang, See-Tong Pourian, Mohammad Rn Johansson, Bjö
Source	Medline; PMC
abstract	BACKGROUND: Androgen‐sensitive prostate cancer cell‐line LNCaP‐FGC and androgen‐resistant line LNCaP‐r constitute a model for development of androgen resistance in prostate cancer. METHODS: Proteins differently expressed in the two cell‐lines were identified by two‐dimensional (2‐D) electrophoresis and mass spectrometry. HSP60, more abundant in LNCaP‐r, was studied by RT‐PCR and immunohistochemistry in specimens of human prostate cancer. RESULTS: HSP60 was upregulated in LNCaP‐r, nm23 in LNCaP‐FGC, and titin (two isoforms) in either LNCaP‐r or LNCaP‐FGC. In non‐malignant prostate, HSP60‐staining was in the glandular compartment, particularly basal epithelial cells. In prostate cancer, most epithelial cells showed moderate‐strong staining without apparent correlation between staining intensity and Gleason grade. CONCLUSIONS: The LNCaP‐FGC/LNCaP‐r model, characterized by 2‐D electrophoresis, reveals distinct proteomic alterations. With HSP60, results from cell‐lines correlated with clinical results, indicating that this model can be used for dissection of mechanisms involved in transformation to androgen resistance and assignment of protein markers in prostate cancer. Prostate © 2006 Wiley‐Liss, Inc.
has issue date	2006-05-16(xsd:dateTime)
bibo:doi	10.1002/pros.20453
bibo:pmid	16705742
has license	no-cc
sha1sum (hex)	1a506d0e759340ff0bb45edfa725675d44134d9d
schema:url	https://doi.org/10.1002/pros.20453
resource representing a document's title	Proteomic comparison of prostate cancer cell lines LNCaP‐FGC and LNCaP‐r reveals heatshock protein 60 as a marker for prostate malignancy
has PubMed Central identifier	PMC7168115
has PubMed identifier	16705742
schema:publication	Prostate
resource representing a document's body	covid:1a506d0e759340ff0bb45edfa725675d44134d9d#body_text
is schema:about of	named entity 'androgen' named entity 'mechanisms' named entity 'prostate cancer' named entity 'resistance' named entity 'Prostate Cancer' named entity 'Protein' named entity 'MECHANISMS' named entity 'ABUNDANT' named entity 'PROTEOMIC' named entity 'REVEALS' named entity 'PROSTATE' named entity 'MARKER' named entity 'REVEALS' named entity 'titin' named entity 'correlated' named entity 'HSP60' named entity 'human' named entity 'development' named entity 'Gleason' named entity 'assignment' named entity 'staining' named entity 'Comparison' named entity 'Marker' named entity 'HSP60' named entity 'prostate' named entity 'RT-PCR' named entity 'Androgen-sensitive' named entity 'isoforms' named entity 'titin' named entity 'staining' named entity 'immunohistochemistry' named entity 'LNCaP' named entity 'prostate cancer' named entity 'proteomic' named entity 'prostate cancer' named entity 'upregulated' named entity 'epithelial cells' named entity 'androgen' named entity '2-D electrophoresis' named entity 'Gleason grade' named entity 'prostate cancer' named entity 'staining' named entity 'Heatshock Protein' named entity 'LNCaP' named entity 'resistance' named entity 'iodoacetamide' named entity 'androgen ablation' named entity 'HSP60' named entity 'protein identification' named entity 'ARPC' named entity 'staining' named entity 'nucleoside diphosphate' named entity 'protein' named entity 'transactivation' named entity 'muscle protein' named entity 'mRNA' named entity 'deamidation' named entity 'Promega' named entity 'multiple-component' named entity 'staining' named entity 'LNCaP' named entity 'kDa' named entity 'acetonitrile' named entity 'higher concentration' named entity 'HSP60' named entity 'quantitative RT-PCR' named entity 'protein' named entity 'HSP60' named entity 'epithelial' named entity 'epithelial cells' named entity 'Student's t-test' named entity 'carcinogenesis' named entity 'proteomic'

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