About: Budded baculovirus particle structure revisited

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Budded baculovirus particle structure revisited
Creator	Rottier, Peter Bosch, Berend-Jan Wang, Qiushi Van Lent, Jan Van Oers, Monique Vlak, Just
Source	Elsevier; Medline; PMC
abstract	Abstract Baculoviruses are a group of enveloped, double-stranded DNA insect viruses with budded (BV) and occlusion-derived (ODV) virions produced during their infection cycle. BVs are commonly described as rod shaped particles with a high apical density of protein extensions (spikes) on the lipid envelope surface. However, due to the fragility of BVs the conventional purification and electron microscopy (EM) staining methods considerably distort the native viral structure. Here, we use cryo-EM analysis to reveal the near-native morphology of two intensively studied baculoviruses, Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and Spodoptera exigua MNPV (SeMNPV), as models for BVs carrying GP64 and F as envelope fusion protein on the surface. The now well-preserved AcMNPV and SeMNPV BV particles have a remarkable elongated, ovoid shape leaving a large, lateral space between nucleocapsid (NC) and envelope. Consistent with previous findings the NC has a distinctive cap and base structure interacting tightly with the envelope. This tight interaction may explain the partial retaining of the envelope on both ends of the NC and the disappearance of the remainder of the BV envelope in the negative-staining EM images. Cryo-EM also reveals that the viral envelope contains two layers with a total thickness of ≈6–7nm, which is significantly thicker than a usual biological membrane (<4nm) as measured by X-ray scanning. Most spikes are densely clustered at the two apical ends of the virion although some envelope proteins are also found more sparsely on the lateral regions. The spikes on the surface of AcMNPV BVs appear distinctly different from those of SeMNPV. Based on our observations we propose a new near-native structural model of baculovirus BVs.
has issue date	2016-02-29(xsd:dateTime)
bibo:doi	10.1016/j.jip.2015.12.001
bibo:pmid	26743500
has license	els-covid
sha1sum (hex)	1038bb44c06e9429393d9a40c07b53d2baf850c9
schema:url	https://doi.org/10.1016/j.jip.2015.12.001
resource representing a document's title	Budded baculovirus particle structure revisited
has PubMed Central identifier	PMC7127228
has PubMed identifier	26743500
schema:publication	Journal of Invertebrate Pathology
resource representing a document's body	covid:1038bb44c06e9429393d9a40c07b53d2baf850c9#body_text
is schema:about of	named entity 'Most' named entity 'lateral' named entity 'apical' named entity 'BACULOVIRUS' covid:arg/1038bb44c06e9429393d9a40c07b53d2baf850c9 named entity 'STRUCTURE' named entity 'ELONGATED' named entity 'OVOID SHAPE' named entity 'CRYO-EM' named entity 'VIRAL ENVELOPE' named entity 'METHODS' named entity 'USUAL' named entity 'STAINING' named entity 'ANALYSIS' named entity 'DOUBLE-STRANDED DNA' named entity 'CONTAINS' named entity 'CONVENTIONAL' named entity 'LAYERS' named entity 'NEAR' named entity 'PARTIAL' named entity 'STRUCTURE' named entity 'TOTAL' named entity 'DUE TO' named entity 'HIGH' named entity 'BACULOVIRUS' named entity 'X-RAY' named entity 'HAVE' named entity 'THEIR' named entity 'USE' named entity 'MEASURED' named entity 'ELECTRON MICROSCOPY' named entity 'PROTEIN ' named entity 'CARRYING' named entity 'NATIVE' named entity 'ROD' named entity 'DESCRIBED' named entity 'HERE' named entity 'ENVELOPE' named entity 'TIGHT' named entity 'SCANNING' named entity 'DISTORT' named entity 'OCCLUSION' named entity 'APPEAR' named entity 'CLUSTERED' named entity 'BIOLOGICAL MEMBRANE' named entity 'FUSION PROTEIN' named entity 'VIRION' named entity 'INTERACTING' named entity 'CAP' named entity 'RETAINING' named entity 'CONSISTENT WITH' named entity 'PARTICLE' named entity 'IMAGES' named entity 'PURIFICATION' named entity 'PRESERVED' named entity 'OUR' named entity 'DISAPPEARANCE' named entity 'NEW' named entity 'ENDS' named entity 'BACULOVIRUSES' named entity 'APICAL' named entity 'DENSITY' named entity 'DERIVED' named entity 'MODELS' named entity 'LARGE' named entity 'OBSERVATIONS' named entity 'EXTENSIONS' named entity 'MODEL OF' named entity 'LEAVING' named entity 'REVEAL' named entity 'FOUND' named entity 'INTERACTION' named entity 'PARTICLES' named entity 'INSECT VIRUSES' named entity 'FRAGILITY' named entity 'ACMNPV'

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