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About:
Pooling of Nasopharyngeal Swab Specimens for SARS-CoV-2 detection by RT-PCR
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covidontheweb.inria.fr
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Academic Article
research paper
schema:ScholarlyArticle
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type
Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
title
Pooling of Nasopharyngeal Swab Specimens for SARS-CoV-2 detection by RT-PCR
Creator
Navarro, David
Albert, Eliseo
Torres, Ignacio
source
MedRxiv
abstract
Systematic testing of large population groups by RT-PCR is mandatory to Covid-19 case identification and contact tracing in order to minimize the likelyhood of resurgence in contagion. Sample pooling for RT-PCR has been effectively used to detect community transmission of SARS CoV-2. Nevertheless, this procedure may decrease the sensitivity of RT-PCR assays due to specimen dilution. We evaluated the efficacy of this strategy for diagnosis of Covid-19 using a sensitive commercially-available RT-PCR targeting SARS CoV-2 E and RdRp genes in a single reaction. A total of 20 mini-pools containing either 5 (n=10) or 10 (n=10) nasopharyngeal exudates collected in universal transport medium were made, each of which including a unique positive NP specimen. Positive specimens yielding CT <32 for the E gene (6 out of 10) or <35.2 for the RdRP gene (7 out of 10) were detected in mini-pools of both sizes. In contrast, most NP samples displaying CTs > 35.8 for the E gene or 35.7 for the RdRP gene remained undetected in mini-pools of 5 specimens (3/4 and 2/3, respectively) or in mini-pools of 10 samples (4/4 and 3/3, respectively.
has issue date
2020-04-25
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bibo:doi
10.1101/2020.04.22.20075598
has license
medrxiv
sha1sum (hex)
0ded103eea098003c2f1188c2b40c3e14180ee65
schema:url
https://doi.org/10.1101/2020.04.22.20075598
resource representing a document's title
Pooling of Nasopharyngeal Swab Specimens for SARS-CoV-2 detection by RT-PCR
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covid:0ded103eea098003c2f1188c2b40c3e14180ee65#body_text
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named entity 'RT-PCR'
named entity 'positive'
named entity 'diagnosis'
named entity 'reaction'
named entity 'effectively'
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