About: Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
Creator	Hordvik, Ivar Lie, Kai Olsvik, Pål Jordal, Ann-Elise Nilsen, Tom
Source	PMC
abstract	BACKGROUND: Salmonid fishes are among the most widely studied model fish species but reports on systematic evaluation of reference genes in qRT-PCR studies is lacking. RESULTS: The stability of six potential reference genes was examined in eight tissues of Atlantic salmon (Salmo salar), to determine the most suitable genes to be used in quantitative real-time RT-PCR analyses. The relative transcription levels of genes encoding 18S rRNA, S20 ribosomal protein, β-actin, glyceraldehyde-3P-dehydrogenase (GAPDH), and two paralog genes encoding elongation factor 1A (EF1A(A )and EF1A(B)) were quantified in gills, liver, head kidney, spleen, thymus, brain, muscle, and posterior intestine in six untreated adult fish, in addition to a group of individuals that went through smoltification. Based on calculations performed with the geNorm VBA applet, which determines the most stable genes from a set of tested genes in a given cDNA sample, the ranking of the examined genes in adult Atlantic salmon was EF1A(B)>EF1A(A)>β-actin>18S rRNA>S20>GAPDH. When the same calculations were done on a total of 24 individuals from four stages in the smoltification process (presmolt, smolt, smoltified seawater and desmoltified freshwater), the gene ranking was EF1A(B)>EF1A(A)>S20>β-actin>18S rRNA>GAPDH. CONCLUSION: Overall, this work suggests that the EF1A(A )and EF1A(B )genes can be useful as reference genes in qRT-PCR examination of gene expression in the Atlantic salmon.
has issue date	2005-11-17(xsd:dateTime)
bibo:doi	10.1186/1471-2199-6-21
bibo:pmid	16293192
has license	cc-by
sha1sum (hex)	0a1533470817bc5ef0d0d0af56386a96b505dc0d
schema:url	https://doi.org/10.1186/1471-2199-6-21
resource representing a document's title	Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
has PubMed Central identifier	PMC1314898
has PubMed identifier	16293192
schema:publication	BMC Mol Biol
resource representing a document's body	covid:0a1533470817bc5ef0d0d0af56386a96b505dc0d#body_text
is schema:about of	named entity 'systematic' named entity 'MODEL' named entity 'QRT-PCR' named entity 'BUT' named entity 'STUDIES' named entity 'FISHES' named entity 'FISH SPECIES' named entity 'GENES' named entity 'MOLECULAR BIOLOGY' named entity 'REAL-TIME RT-PCR' named entity 'ATLANTIC SALMON' named entity 'GENES' named entity 'POTENTIAL' named entity 'EVALUATION' named entity 'LACKING' named entity 'SALMONID' named entity 'REFERENCE' named entity 'REPORTS' named entity 'BACKGROUND' named entity 'BMC' named entity 'STUDIES' named entity 'REFERENCE' named entity 'EVALUATION' named entity 'Palo Alto' named entity 'absorbance' named entity 'physiological stress' named entity 'reference genes' named entity 'qRT-PCR' named entity 'intestine' named entity 'thymus' named entity 'Agilent' named entity 'RNase' named entity 'smoltification' named entity 'absorbance' named entity 'qPCR' named entity 'EF1A' named entity 'mRNA' named entity 'GAPDH' named entity 'eEF1A' named entity 'eEF1A' named entity 'β-actin' named entity 'PCR' named entity 'isoforms' named entity 'gene' named entity 'cDNA' named entity 'PCR' named entity 'β-actin' named entity 'paralog' named entity 'liver' named entity 'reference genes' named entity 'A260' named entity 'thymus' named entity 'RNA extraction' named entity 'reference genes' named entity 'kidney' named entity 'morphological' named entity '18S rRNA' named entity 'thymus' named entity 'smoltification' named entity 'gene' named entity 'β-actin' named entity 'mRNA expression' named entity 'EF1A' named entity 'expression levels' named entity 'ribosomal RNA' named entity 'A260' named entity 'PCR primers' named entity 'ribosomal RNA' named entity 'smoltification' named entity 'physiological' named entity 'DNase' named entity '18S rRNA gene' named entity '2, 3' named entity 'β-actin' named entity 'β-actin' named entity 'reference genes' named entity 'RNA'

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