About: Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages
Creator	Mori, Masahumi Ono, Masaaki Shibata, Isao Sueyoshi, Masuo Tsuda, Tomoyuki Uruno, Katsuyoshi
Source	Elsevier; Medline; PMC
abstract	This paper describes the isolation of porcine epidemic diarrhea (PED) virus in Vero and porcine cell cultures, and the influence of age on disease in experimental infection. PED virus was isolated from the small intestine of piglets inoculated with PED samples and cultured in Vero, porcine bladder and kidney cells propagated in collagen-coated tissue culture plates in maintenance medium (MM) containing trypsin. In porcine bladder and kidney cell cultures inoculated with isolated PED virus, cytopathic effects (CPE) including cell fusion were detected. Specific brilliant fluorescence was observed in the cytoplasm of these cells. Two- and 7-day old, and 2-, 4-, 8- and 12-week old specific pathogen-free (SPF) pigs were orally inoculated with PED virus isolated from an outbreak. All 2- and 7-day old pigs inoculated developed severe watery diarrhea from post-inoculation day (PID) 1 and died between PID 3 and 4. Although three of five 2-week old pigs developed diarrhea on PID 1–4, they eventually recovered. In the 4-week old group, three of five pigs had mild diarrhea for 1–2 days. None of the 8- and 12-week old pigs showed any clinical signs. Antibodies against PED virus were detected in all surviving pigs by virus neutralization (VN) test and immunofluorescence assay (IFA). Therefore, there is an age-dependent resistance to pathogenic PED virus infection in pigs.
has issue date	2000-03-15(xsd:dateTime)
bibo:doi	10.1016/s0378-1135(99)00199-6
bibo:pmid	10727829
has license	no-cc
sha1sum (hex)	089ef564e79dce7d074a35b939cda4b1c59da347
schema:url	https://doi.org/10.1016/s0378-1135%2899%2900199-6
resource representing a document's title	Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages
has PubMed Central identifier	PMC7117361
has PubMed identifier	10727829
schema:publication	Vet Microbiol
resource representing a document's body	covid:089ef564e79dce7d074a35b939cda4b1c59da347#body_text
is schema:about of	named entity 'cytoplasm' named entity 'PID' named entity 'Antibodies' named entity 'propagated' named entity 'inoculated' named entity 'PED' named entity 'diarrhea' named entity 'week' named entity 'infection' named entity 'ages' named entity 'AGES' named entity 'PATHOGEN' named entity 'ASSAY' named entity 'PED' named entity 'OBSERVED' named entity 'DIARRHEA' named entity 'ISOLATED' named entity 'SEVERE' named entity 'BLADDER' named entity 'OLD' named entity 'DEPENDENT' named entity 'KIDNEY' named entity 'KIDNEY CELL' named entity 'CYTOPATHIC EFFECTS' named entity 'COLLAGEN' named entity 'B14' named entity 'FREE' named entity 'TISSUE CULTURE' named entity 'ORALLY' named entity 'CONTAINING' named entity 'POST' named entity 'INCLUDING' named entity 'SMALL INTESTINE' named entity 'DAY' named entity 'AGE' named entity 'VERO' named entity 'CULTURE PLATES' named entity 'MILD' named entity 'SAMPLES' named entity 'CELL CULTURES' named entity 'MAINTENANCE' named entity 'EXPERIMENTAL INFECTION' named entity 'ISOLATION' named entity 'CLINICAL SIGNS' named entity 'PIGS' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'MEDIUM' named entity 'RECOVERED' named entity 'CELLS' named entity 'AEC' named entity 'CELL CULTURES' named entity 'ISOLATION' named entity 'VIRUS INFECTION' named entity 'VIRUS' named entity 'PAPER' named entity 'GROUP' named entity 'CYTOPLASM' named entity 'B12' named entity 'DIFFERENT' named entity 'PORCINE' named entity 'WATERY DIARRHEA' named entity 'PATHOGENIC' named entity 'DISEASE' named entity 'CULTURED' named entity 'ANTIBODIES' named entity 'PORCINE' named entity 'PIGS' named entity 'PID' named entity 'DIED' named entity 'VIRUS NEUTRALIZATION' named entity 'DAYS' named entity 'THESE' named entity 'INOCULATION' named entity 'COATED' named entity 'PORCINE EPIDEMIC DIARRHEA' named entity 'EXPERIMENTAL INFECTION'

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