About: Evaluation of a PCR-electrospray ionization mass spectrometry platform for detection and identification of fungal pathogens directly from prospectively collected bronchoalveolar lavage specimens

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About: Evaluation of a PCR-electrospray ionization mass spectrometry platform for detection and identification of fungal pathogens directly from prospectively collected bronchoalveolar lavage specimens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Evaluation of a PCR-electrospray ionization mass spectrometry platform for detection and identification of fungal pathogens directly from prospectively collected bronchoalveolar lavage specimens
Creator	Metzgar, David Rothman, Richard Lovari, Robert Massire, Christian Carolan, Heather Jia, Bei Miller, Heather Toleno, Donna Blyn, Lawrence D'alessio, Franco Zhang, Sean
source	Elsevier; Medline; PMC
abstract	Abstract The incidence of invasive fungal infections is on the rise worldwide due to the growth of the immunocompromised population. We report here the use of a diagnostic assay that utilizes a universal extraction method, broad spectrum PCR amplification and analysis via electrospray ionization mass spectrometry (PCR/ESI-MS) to detect and identify more than 200 pathogenic fungi directly from bronchoalveolar lavage (BAL) specimens in less than 8 hours. In this study, we describe both analytical and clinical performance of the assay, when run with prospectively collected clinical BAL specimens. In 146 patients with probable and possible fungal infections defined by EORTC/MSG (European Organization for Research and Treatment of Cancer/Mycoses Study Group) criteria, the PCR/ESI-MS assay demonstrated a sensitivity of 90.9% (95% CI: 76.4–96.9%) and a specificity of 82.3% (95% CI: 74.2–88.2%). This data demonstrates the utility of a non-culture based broad fungal targets molecular diagnostic tool for rapid and accurate diagnosis of invasive fungal infections in patients at risk of developing fungal diseases.
has issue date	2020-01-15(xsd:dateTime)
bibo:doi	10.1016/j.diagmicrobio.2020.114988
bibo:pmid	32107092
has license	els-covid
sha1sum (hex)	059dda4ff1bee5fa590fec885819e4761c0fd085
schema:url	https://doi.org/10.1016/j.diagmicrobio.2020.114988
resource representing a document's title	Evaluation of a PCR-electrospray ionization mass spectrometry platform for detection and identification of fungal pathogens directly from prospectively collected bronchoalveolar lavage specimens
has PubMed Central identifier	PMC7127232
has PubMed identifier	32107092
schema:publication	Diagn Microbiol Infect Dis
resource representing a document's body	covid:059dda4ff1bee5fa590fec885819e4761c0fd085#body_text
is schema:about of	named entity 'utility' named entity 'population' named entity 'rise' named entity 'bronchoalveolar lavage' named entity 'ionization' named entity 'DETECTION' named entity 'STUDY' named entity 'MORE THAN' named entity 'TREATMENT' named entity 'USE OF' named entity 'PATHOGENIC FUNGI' named entity 'RAPID' named entity 'TO DETECT' named entity 'EORTC' named entity 'SENSITIVITY' named entity 'FUNGI ' named entity '8 HOURS' named entity 'UTILITY' named entity 'COLLECTED' named entity 'INVASIVE FUNGAL INFECTIONS' named entity 'TARGETS' named entity 'COLLECTED' named entity 'BRONCHOALVEOLAR LAVAGE' named entity 'IDENTIFICATION' named entity 'DIRECTLY' named entity 'PCR' named entity 'SPECIMENS' named entity 'PLATFORM' named entity 'EVALUATION' named entity 'UNIVERSAL' named entity 'DIAGNOSIS' named entity 'BROAD SPECTRUM' named entity 'ORGANIZATION' named entity 'REPORT' named entity 'WORLDWIDE' named entity 'HERE' named entity 'ANALYSIS' named entity 'CULTURE' named entity 'EXTRACTION METHOD' named entity 'PCR AMPLIFICATION' named entity 'ANALYTICAL' named entity 'BASED' named entity 'RUN' named entity 'POPULATION' named entity 'FUNGAL PATHOGENS' named entity 'ELECTROSPRAY IONIZATION MASS SPECTROMETRY ' named entity 'IDENTIFY' named entity 'RISK OF' named entity 'SPECIMENS' named entity 'POSSIBLE' named entity 'FUNGAL INFECTIONS' named entity 'PERFORMANCE' named entity 'LESS THAN' named entity 'CRITERIA' named entity 'PROBABLE' named entity 'AT RISK' named entity 'ACCURATE' named entity 'DIAGNOSTIC ASSAY' named entity 'STUDY GROUP' named entity '200' named entity 'BAL' named entity 'DEMONSTRATED' named entity 'PCR' named entity 'DUE TO' named entity 'FOR RESEARCH' named entity 'PATIENTS' named entity 'DESCRIBE' named entity 'INCIDENCE' named entity 'FUNGAL DISEASES' named entity 'DEFINED' named entity 'SPECIFICITY'

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