About: A novel method to produce armored double-stranded DNA by encapsulation of MS2 viral capsids

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About: A novel method to produce armored double-stranded DNA by encapsulation of MS2 viral capsids Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A novel method to produce armored double-stranded DNA by encapsulation of MS2 viral capsids
Creator	Sun, Yu Zhang, Lei Zhang, Rui Li, Jinming Zhang, Kuo Jia, Tingting Wang, Guojing Le Chang, &
Source	Medline; PMC
abstract	With the rapid development of molecular diagnostic techniques, there is a growing need for quality controls and standards with favorable properties to monitor the entire detection process. In this study, we describe a novel method to produce armored hepatitis B virus (HBV) and human papillomavirus (HPV) DNA for use in nucleic acid tests, which was confirmed to be stable, homogeneous, noninfectious, nuclease resistant, and safe for shipping. We demonstrated that MS2 bacteriophage could successfully package double-stranded DNA of 1.3-, 3-, 3.5-, and 6.5-kb length into viral capsids with high reassembly efficiency. This is the first application of RNA bacteriophage MS2 as a platform to encapsulate double-stranded DNA, forming virus-like particles (VLPs) which were indistinguishable from native MS2 capsids in size and morphology. Moreover, by analyzing the interaction mechanism of pac site and the MS2 coat protein (CP), we found that in addition to the recognized initiation signal TR-RNA, TR-DNA can also trigger spontaneous reassembly of CP dimers, providing a more convenient and feasible method of assembly. In conclusion, this straightforward and reliable manufacturing approach makes armored DNA an ideal control and standard for use in clinical laboratory tests and diagnostics, possessing prospects for broad application, especially providing a new platform for the production of quality controls for DNA viruses. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00253-015-6664-4) contains supplementary material, which is available to authorized users.
has issue date	2015-05-19(xsd:dateTime)
bibo:doi	10.1007/s00253-015-6664-4
bibo:pmid	25981999
has license	no-cc
sha1sum (hex)	02cadf8addb72d55755378a1780b0eebccddb885
schema:url	https://doi.org/10.1007/s00253-015-6664-4
resource representing a document's title	A novel method to produce armored double-stranded DNA by encapsulation of MS2 viral capsids
has PubMed Central identifier	PMC7079959
has PubMed identifier	25981999
schema:publication	Appl Microbiol Biotechnol
resource representing a document's body	covid:02cadf8addb72d55755378a1780b0eebccddb885#body_text
is schema:about of	named entity 'manufacturing' named entity 'armored' named entity 'MS2' named entity 'high' named entity 'reliable' named entity 'signal' named entity 'nucleic acid' named entity 'ENCAPSULATION' named entity '1.3' named entity 'MS2' named entity 'SPONTANEOUS' named entity 'STUDY' named entity 'GROWING' named entity 'RNA' named entity 'PAC SITE' named entity 'SIGNAL' named entity 'DIMERS' named entity 'METHOD' named entity 'NUCLEIC ACID' named entity 'APPLICATION' named entity 'RELIABLE' named entity 'MECHANISM' named entity 'CAPSIDS' named entity 'MONITOR' named entity 'STANDARDS' named entity 'SAFE' named entity 'PLATFORM' named entity 'NEW' named entity 'HOMOGENEOUS' named entity 'USE' named entity 'PROTEIN ' named entity 'FEASIBLE' named entity 'NONINFECTIOUS' named entity 'DESCRIBE' named entity 'VIRAL' named entity 'BROAD' named entity 'DEVELOPMENT' named entity 'IDEAL' named entity 'ASSEMBLY' named entity 'DNA' named entity 'TO PRODUCE' named entity 'IS A' named entity 'DOUBLE-STRANDED DNA' named entity 'INITIATION' named entity 'METHOD' named entity 'VIRAL' named entity 'NOVEL' named entity 'APPROACH' named entity 'MANUFACTURING' named entity 'HUMAN PAPILLOMAVIRUS ' named entity 'DEMONSTRATED' named entity 'STABLE' named entity 'LENGTH' named entity 'EFFICIENCY' named entity 'PACKAGE' named entity 'CONTROL' named entity 'MORPHOLOGY' named entity 'DOUBLE-STRANDED DNA' named entity 'NUCLEASE' named entity 'BACTERIOPHAGE' named entity 'FAVORABLE' named entity 'RAPID' named entity 'PROPERTIES' named entity 'RESISTANT' named entity 'CONFIRMED' named entity 'TESTS' named entity 'ANALYZING' named entity 'VIRUS-LIKE PARTICLES' named entity 'INTERACTION' named entity 'ADDITION' named entity 'DIAGNOSTICS' named entity 'NOVEL' named entity 'TO PRODUCE' named entity 'FORMING'

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